VECTOR CORE

细胞和组织成像核心（核心C）。 该项目的重点是局部表达的影响ofcytokines，和他们的影响， 肿瘤微环境这需要研究转导细胞的递送效率，以及多个转导细胞的递送效率。 这些细胞对肿瘤的影响的不同分析。项目的核心问题是：1）如何有效地 将细胞输送到肿瘤中2)这些细胞是否表达了适当的信息和蛋白质产物？3)是 有什么治疗效果吗后者包括研究细胞浸润、血管生成、肿瘤 衰老和细胞向肿瘤环境的募集。在每种情况下，这些研究都将严格依赖于 定量光学方法学生物成像中心将提供这项核心服务， 设计时就考虑到了这一功能。它配备了一个连续的光学方法，包括所有类型的 光学显微镜和电子显微镜对这个项目至关重要。在本项目范围内， 核心的目标将是多种多样的。例如，我们将量化和表征肿瘤中的细胞浸润 微观环境。我们将确定外源转染的细胞是否达到肿瘤靶点，定量它们的表达。 丰度，确定这些细胞是否表达转导的细胞因子， 由这些细胞因子的表达引起。为了执行这些功能，我们将使用各种各样的 显微技术。在光学显微镜水平，这些包括：组织学，免疫组织学，原位 杂交、激光共聚焦、双光子和活细胞技术。此外，我们在以下方面的丰富经验 计算机化的图像处理和形态测量将允许对观察到的现象进行定量分析， 证实了早些时候，可能相当微妙的质的变化。该核心将被所有项目广泛使用， 尽管所使用的成像工具将因项目而异。在以前提交的提案中， 核心被评为上级，很好地融入了计划，并提供基本的综合服务， 节目因此，本申请中的变化很少，并在引言中进行了描述。

Cell and tissue imaging core (Core C). This program project focuses on the effects of locoregional expression ofcytokines, and their effect on the tumor microenvironment. This demands study of the efficiency of delivery of tranduced cells, and multiple different analyses of the effects of these cells on the tumor. Central questions in projects are: 1) how effectively are cells delivered to the tumor? 2) Is the appropriate message and the protein product expressed by these cells? 3) is any therapeutic effect seen? This latter component includes study of cellular infiltration, angiogcnesis, tumor senescence, and cellular recruitment to the tumor environment. In each case these studies will rely critically on quantitative optical methodologies. The Center for Biolo_c lmaging, in which this core service will be performed, is designed with this function in mind. It is equipped to perform a continuum of optical methods including all types of light and electron microscopy essential to this program project. Within the scope of this project the principal goals of the core will be various. For example we will quantify and characterize cellular infiltrates into the tumor micro-environment. We will determine whether exogenously transfected cells reach tumor targets, quantify their abundance, determine whether these cells are expressing the transduced cytokines, and examine structural changes resulting from the expression of these cytokines. To perform these functions we will use a diverse array of microscopy technologies. At the light microscopic level these include: histological, immuno-histological, in situ hybridization, laser confocal, 2 photon and live cell technologies. Furthermore, our considerable experience in computerized image processing and morphometry will allow quantitative analysis of observed phenomena to corroborate earlier, possibly quite subtle qualitative changes. This core will be used extensively by all projects, though the imaging tools used will vary from project to project. In previous submissions of the proposal, this core was rated as superior, well integrated into the program and offering essential and integrated services to the program. As such the changes in this application are few, and are described in the introduction.