Animal Models Core

动物模型核心设施的目的是支持和集中某些方面的工作 提出的每一个项目。我们认为前列腺癌生物学最好在动物模型系统中进行研究。 允许对进展事件进行实验监测，如转移和雄激素非依赖性 进步。该设施汇集了小鼠遗传学和人类肿瘤异种技术的专业知识，将 允许在最先进的模型系统中解决基本的分子生物学问题 与临床情况的相关性。它还为翻译研究问题提供了一个机会 在相关的临床前模型中立即解决。核心由六个不同的组件组成，它们 将允许基于生物学的假说在合适的动物模型或人类前列腺癌中进行测试 异种移植物。 这一核心的具体目标如下： 1.为五个研究项目提供SCID小鼠 2.在SCID上种植四种人前列腺癌移植瘤：LAPC4、LAPC9、CWR22和LUCAP 老鼠在孢子中寻找调查对象。 3.维持模型小鼠，如Pten和COX-2条件基因敲除，AKT和Myc转基因小鼠 以及每个项目所需的记者专线。 4.为体内实验提供技术支持，如收获异种移植，测定PSA水平， 并为临床前试验注射前药物。 5.提供动物实验和外科手术技术方面的专业知识/咨询 建议进行的研究。 6.为新模式的产生提供专业知识/咨询。

The purpose of the Animal Models Core facility is to support and centralize certain aspects of the work in each project proposed. It is our belief that prostate cancer biology is best studied in animal model systems that allow experimental monitoring of progression events such as metastasis and androgen-independent progression. This facility brings together expertise in mouse genetics and human tumor xenografs that will allow basic molecular biological questions to be addressed in state-of-the art model systems with direct relevance to the clinical situation. It also offers an opportunity for translational research questions to be addressed immediately in relevant pre-clinical models. The Core consists of six distinct components which will allow biologically based hypotheses to be tested in Suitable animal models or human prostate cancer xenografts. The Specific Aims of this Core are as follows: 1. To provide SCID mice to the five research projects 2. To propagate four human prostate cancer xenografts: LAPC4, LAPC9, CWR22, and LUCAP on SCID mice for Investigators in the SPORE. 3. To maintain model mice, such as Pten and Cox-2 conditional knock-out, AKT and Myc transgenic mice as well as reporter lines needed for each project. 4. To provide technical assistance for in vivo experiments, such as harvest xenografts, measure PSA level, and inject pro-drugs for pre-clinical trials. 5. To provide expertise/consultation on animal experimentation and surgical techniques required for the proposed studies. 6. To provide expertise/consultation for the generation of new models.