Amnis ImageStream Cell Analysis System

描述（由申请人提供）：此共享仪器赠款提案用于购买Amnis ImageStream仪器，该仪器将流式细胞术的功能与流式细胞术中细胞的高分辨率数字图像捕获相结合。ImageStream能够检测四种颜色的荧光，并捕获每个细胞的明场和暗场图像。ImageStream不会取代任何现有的流式细胞仪或成像设备，而是提供了一种补充技术，将大大促进资助参与者在应用中的研究，例如混合细胞群中的核易位研究，细胞凋亡，细胞周期和有丝分裂研究，标记物共定位，细胞内运输，细胞：细胞相互作用，荧光原位杂交和基因表达/共定位。除仪器外，还需要一个ImageStream稳定台。需要提供强大数据管理功能的戴尔工作站，以及IDEAS软件的多用户软件许可证。新的ImageStream将被安置在新泽西医学院的流式细胞术核心设施中，并将由经验丰富的流式细胞术技术员Angelika Batista女士操作和维护。首席研究员帕特里夏·菲茨杰拉德-博卡尔斯利博士将与教师咨询委员会协商，提供行政和科学监督。ImageStream将主要由来自NJMS不同部门和生物医学科学学科的13名高产科学家组成的申请小组使用。它们包括：1）Azzam，Edouard -从辐射到非辐射细胞的损伤信号传导; 2）Fitzgerald-Bocarsly，Patricia -HIV发病机制中的浆细胞样树突细胞; 3）Gause，William -体内免疫应答期间的细胞因子基因表达; 4）豪厄尔，Roger -放射性的非均匀分布的影响; 5）Kotenko，Sergei -干扰素β das在抗病毒应答中的作用; 6）Lambert，Muriel -范可尼贫血A组中的DNA修复缺陷; 7）Leibovich，Joseph -Toll样受体、腺苷和血管生成; 8）Lukac，大卫-KSHV裂解开关蛋白的DNA结合机制和KSHV基因表达的动力学; 9）Rameschwar，Pranela -炭疽和新兴免疫系统的疫苗障碍;神经免疫-间充质干细胞轴中的IFN-γ;和乳腺癌-骨髓基质相互作用; 10）Rohowsky-Kochan，克莉丝汀-自体反应性T细胞的细胞因子分泌谱; 11）Salgame，Padmini -结核病中Th 1免疫的诱导; 12）Studzinski，乔治-维生素D类似物作为癌症化疗中的佐剂;和13）Molina，卡洛斯-黑色素瘤中ICER的Ras介导的蛋白酶体降解。预计这组研究人员将利用几乎100%的仪器总使用量。剩余的仪器时间将分配给NIH资助的研究者或纽瓦克校区的其他个人。当ImageStream未以其他方式使用时，可不时向非UMDNJ研究者提供仪器时间。

DESCRIPTION (provided by applicant): This Shared Instrument Grant proposal is for the purchase of an Amnis ImageStream instrument that combines the power of flow cytometry with the capture of high resolution digital images of cells in flow. The ImageStream is able to detect four colors of fluorescence as well as capture brightfield and darkfield imagery of each cell. The ImageStream does not replace any existing flow cytometric or imaging equipment, but rather, provides a complementary technology that will greatly facilitate the research of the grant participants in applications such as nuclear translocation studies in mixed populations of cells, apoptosis, cell cycle and mitosis studies, marker co-localization, intracellular trafficking, cell:cell interactions, fluorescent in situ hybridization and gene expression/co-localization. In addition to the instrument, a stabilizing table for the ImageStream is also requested. A Dell Workstation that provides powerful data management is requested, as is a multi-user software license for the IDEAS software. The new ImageStream will be housed in the Flow Cytometry Core Facility at the New Jersey Medical School and will be operated and maintained by an experienced flow cytometry technician Ms. Angelika Batista. The Principal Investigator, Dr. Patricia Fitzgerald-Bocarsly will provide administrative and scientific oversight in consultation with a Faculty Advisory Committee. The ImageStream will be primarily utilized by the applicant group of thirteen highly-productive scientists from diverse departments and biomedical science disciplines at the NJMS. They include: 1) Azzam, Edouard - Damage signaling from irradiated to non-irradiated cells; 2) Fitzgerald-Bocarsly, Patricia - Plasmacytoid dendritic cells in HIV pathogenesis; 3) Gause, William - Cytokine gene expression during in vivo immune response; 4) Howell, Roger - Effects of nonuniform distributions of radioactivity; 5) Kotenko, Sergei - Role of interferon lambdas in antiviral response; 6) Lambert, Muriel - DNA repair defect in Fanconi Anemia Group A; 7) Leibovich, Joseph - Toll-like receptors, adenosine and angiogenesis; 8) Lukac, David - DNA binding mechanism of the KSHV lytic switch protein and kinetics of KSHV gene expression; 9) Rameschwar, Pranela - Vaccine hurdle to anthrax and the emerging immune system; IFN-gamma in the neuroimmune-mesenchymal stem cell axis; and Breast cancer-bone marrow stromal interactions; 10) Rohowsky-Kochan, Christine - Cytokine secretion profile of autoreactive T cells; 11) Salgame, Padmini - Induction of Th1 immunity in tuberculosis; 12) Studzinski, George - Vitamin D analogs as adjuvants in chemotherapy of cancer; and 13) Molina, Carlos - Ras-Mediated proteasomal degradation of ICER in melanomas. This group of investigators is anticipated to utilize almost 100% of the total usage of the instrument. Remaining instrument time will be allocated to NIH-funded investigators or other individuals at the Newark campus. Instrument time may be made available to non-UMDNJ investigators from time-to-time when the ImageStream is not otherwise being used.