Acquisition of a Bruker E580 Pulse EPR Spectrometer

描述(申请人提供)：需要资金购买一台布鲁克ELEXSYS580脉冲光谱仪，能够在低温下运行双电子共振(DER)、双量子相干(DQC)和电子核双共振(Endor)实验。威斯康星医学院(MCW)或周边地区社区都没有这种脉搏仪器。脉冲EPR谱在商业仪器和应用方面的最新进展使这项技术迅速成为定点自旋标记(SDSL)和金属EPR研究的关键技术。鹿和DQC方法在15-50°范围内测量旋转间距离的能力为研究具有生物医学意义的研究问题开辟了一种全新的可能性范围。 五个主要用户将利用该仪器测量蛋白质或多肽系统内的远距离范围。此外，还确定了许多次要用户，每个用户都需要该地区没有的先进脉搏仪器。每个主要用户都是由美国国立卫生研究院资助的，并拥有一个致力于蛋白质结构和功能动力学进步的成熟且不断壮大的研究小组。除了改善周边社区的研究环境外，这一工具还将立即显著提高本提案中概述的项目的生产率。 拥有这种仪器将提供的脉冲能力对于讨论的NIH资助项目的快速进展以及为MCW研究人员和参观者提供最先进的EPR仪器至关重要。

DESCRIPTION (provided by applicant): Funds are requested to purchase a Bruker ELEXSYS 580 pulse spectrometer capable of running double electron electron resonance (DEER), double quantum coherence (DQC), and electron nuclear double resonance (ENDOR) experiments at cryogenic temperatures. Pulse instrumentation of this kind is not available at the Medical College of Wisconsin (MCW), or in the surrounding regional community. Recent advances in the commercial instrumentation and application of pulsed EPR spectroscopy are rapidly making this technique essential to the site-directed spin labeling (SDSL) and metal EPR research communities. The ability of DEER and DQC methods to measure interspin distances in the range of 15-50¿ opens up an entirely new range of possibilities in the study of biomedically significant research problems. Five major users will utilize this instrument to measure long distance ranges within protein or peptide systems. In addition, numerous minor users are identified, each with a need for advanced pulse instrumentation not available in the region. Each major user is NIH-funded and has an established and growing research group dedicated to the advancement of protein structure and functional dynamics. This instrumentation will immediately and significantly advance the productivity of the projects outlined in this proposal in addition to enhancing the research environment for the surrounding community. Having the pulse capability that this instrumentation will provide is vital to the rapid progress of the discussed NIH-funded projects as well as to providing MCW researchers and visitors access to state-of-the-art EPR instrumentation.