BD FACSARIA SYSTEM - FLOW CYTOMETER

描述(申请人提供)：多年来，放射肿瘤学中的生物学研究广泛使用了流式细胞仪分析。华盛顿大学放射肿瘤学系的我们也不例外。1978年，我们安装了第一台流式细胞仪。自那以后，我们使用流式细胞仪进行了大量研究，并在随后的几年中对应用的设备进行了微小的改进。然而，随着分子生物学在放射肿瘤学研究中的应用日益受到重视，以及荧光探针技术的巨大进步，该站点目前可用的系统已经过时，不足以进行所需的实验。这项提议的目的是申请资金，用于采购一台流式细胞仪/分选机，以取代华盛顿大学医学院放射肿瘤学系目前可用的系统。我们申请资金的系统是由BD生物科学公司制造的FACSAria。应要求，该系统具备开展该部现有和未来研究人员以及一直使用我们设施的其他部门调查人员提出的研究所需的所有功能。拥有三个激发波长和最多9个荧光波长的能力，使我们能够进行实验，在其中可以同时分析每个细胞中几个分子和细胞参数的关系。此外，该系统的激光激发和荧光检测配置需要较低的荧光激发功率，从而减少对细胞的损害，这些细胞被分选并随后进行继代培养或检测对此类损伤敏感的分子生物学参数。放射肿瘤学生物学实验室距离医学院主校区很远。它是医学院校园里唯一可以立即接触到电离放射源的流式细胞仪设备，这是我们研究的一个主要组成部分。与流式细胞仪设施毗邻的湿式实验室和组织培养设施，使这款新型流式细胞仪能够灵活地进行医学院校园内其他流式细胞仪实验室无法提供的实验。

Description (provided by applicant): Biology research in radiation oncology has extensively utilized flow cytometric analyses over the years. We, at the Radiation Oncology Department of Washington University, are no exception. We have our first flow cytometer installed in 1978. We have since conducted numerous studies using flow cytometry, with minor improvements on the equipment applied during the ensuing years. With emerging emphasis in the application of molecular biology in radiation oncology research and vast advancement in fluorescent probe technology, however, the system currently available at this site has become outdated and insufficient to conduct desired experiments. The objective of this proposal is to request funds for the procurement of a flow cytometer/sorter to replace the currently available system at the Department of Radiation Oncology at Washington University School of Medicine. The system we are requesting funds for is FACSAria manufactured by BD Biosciences. The system as requested has all the features that are required to conduct research proposed by the current and future researchers in the Department as well as investigators from other departments who has been using our facility. Having three excitation wavelength and the capability to detect up to 9 fluorescence wavelengths allow us to conduct experiments in which relationship of several molecular and cellular parameters in each cell can be simultaneously assayed. Additionally the configuration of laser excitation and fluorescence detection for this system requires lower power for fluorochrome excitation, resulting in less damage to cells that are sorted and subsequently subcultured or assayed for molecular biology parameters that are sensitive to such insults. The Radiation Oncology biology laboratory is remotely located from the main campus of the Medical School. It is the only flow cytometry facility on the Medical School campus that has immediate access to ionizing radiation sources, a major component in our research. With the availability of wet laboratory and tissue culture facility also adjacent to the flow cytometry facility, this new flow cytometer offers flexibility in conducting experiments that cannot be obtained from other flow cytometry laboratories on the Medical School campus.