ARRHYTHMIAS IN DOUBLE KNOCKOUT MICE W/ CORONARY ATHEROSCLEROSIS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。麻省理工学院生物学B00克里格实验室。基于两个基因的突变，建立了小鼠动脉粥样硬化遗传模型，一个基因编码SR-BI，这是吸收高密度脂蛋白的重要分子，另一个基因编码apoE，这是低密度脂蛋白代谢的必要分子。这种所谓的“双敲除”模型产生的症状比以前的“单敲除”模型更接近于人类的动脉粥样硬化，后者只改变了编码apoE的DNA。具体来说，双基因敲除小鼠的特征是富含脂质的冠状动脉闭塞、多发性心肌梗死、心脏增大、射血分数和收缩力降低、心电图异常和过早死亡。对这些小鼠的心电图进行详细的研究将为组织学研究和疾病趋势分析提供基础。我们正在为克里格实验室开发一个联网的心电数据收集、管理和实时分析系统。项目最初的范围仅限于心电数据的采集、管理和实时远程互联网监测的硬件和软件设计。该系统包括：a)带滤波器和a /D转换的16通道心电前置放大器；b) Hermes RAID服务器；i)为生物实验室技术人员提供基于web的GUI界面。ii.)将输入的心电数据从A/D卡流式传输到磁盘，提供数据的中央网络存档。iii.)在一个多播IP地址上传输加密的心电数据，以便通过互联网进行远程监控。c) Hermes客户端-客户端软件使用GUI界面收集、解密和显示来自服务器的实时数据广播。该软件还允许用户查看驻留在RAID上的存档文件，从而对ECG数据进行回顾性分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Krieger Lab in MITs Biology B00. has developed a murine genetic model for atherosclerosis based on the mutation of two genes one encoding SR-BI, a vital molecule for absorption of HDL, and the other encoding apoE, a requisite molecule for the metabolism of LDL. This so-called "double-knockout" model produces symptoms more closely resembling atherosclerosis in humans than former "single-knockout" models, which only altered DNA encoding apoE. Specifically, double-knockout mice are characterized by lipid-rich coronary artery occlusions, multiple myocardial infarctions, an enlarged heart, reduced ejection fraction and contractility, ECG abnormalities and premature death. Detailed studies of the electrocardiogram (ECG) of these mice will provide a foundation for histological studies and disease trend analysis. We are developing a networked ECG data collection, management, and real-time analysis system for the Krieger Lab. The initial scope of the project has been restricted to the design of hardware and software for the collection, management, and real-time remote internet monitoring of ECG data. The system includes: a) a 16-channel ECG preamplifier with filters and A/D conversion b) a Hermes RAID server that: i.) provides web-based GUI inter face for biology lab technicians. ii.) streams incoming ECG data from the A/D card to disk, providing a central networked archive of the data. iii.) streams encrypted ECG data on a multicast IP address for remote monitoring via the internet. c) Hermes client - Client software collects, decrypts and displays the real-time data broadcasts from the server using a GUI interface. The software also permits retrospective analysis of ECG data by allowing the user to view archived files residing on the RAID.