MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

蛋白质和细胞器的适当分布是真核细胞的基本属性。这种“两极” 允许它们经历分裂、极化生长和分化等过程。有一种方法可以让你 建立极性是通过引导膜的运输。这种调节是一个保守的过程，从酵母到 因此，这种现象如何发生的基本原理可以在酵母中发现，然后在 在动物模型中有更多细节。我建议研究磷脂酰肌醇-4-磷酸(PI4P)在 分泌，特别是评估其在Myo2p活动中的作用。Myo2p是一种马达蛋白，负责 在细胞周期中几种细胞成分的运动和分离。它的货物之一是 源自高尔基体的分泌囊。大量证据表明，PI4P是主要的PI 此外，我们有初步的遗传和成像数据表明，PI4P可能在 在依赖Myo2p的分泌性小泡运输中的作用。我计划通过各种基因来进一步研究这一点 技术，即过表达抑制分析、突变体的产生和鉴定等。 此外，使用酵母双杂交和常规生化分析的改进版本，我将尝试 确定难以捉摸的分泌囊Myo2受体。我的结果，以及实验室里的其他人和 在其他地方，这将有助于我们理解Myo2p是如何被调控的，这对细胞的生存至关重要。

Proper distribution of proteins and organelles is an essential property of eukaryotic cells. This "polarity" allows them to undergo processes like division, polarized growth, and differentiation. One way you can establish polarity is by directing membrane traffick. This regulation is a conserved process from yeast to mammals, so the basic principles of how that is occurring can be uncovered in yeast and then studied in more detail in animal models. I propose to study the role of phosphatidylinositol-4-phosphate (PI4P) in secretion, specifically, to evaluate its role in Myo2p activity. Myo2p is the motor protein responsible for the movement and segregation of several cellular components during the cell cycle. One of its cargoes is the secretory vesicles, that originate from the Golgi. Abundant evidence indicate that PI4P is the major PI species in the Golgi, plus we have preliminary genetic and imaging data suggesting that PI4P may play a role in Myo2p-dependent secretory vesicle transport. I plan to investigate this further by various genetic techniques, namely overexpression suppression analysis, generation and characterization of mutants,etc. Also, using modified versions of the yeast two-hybrid and conventional biochemical assays, I will try to identify the elusive secretory vesicle Myo2-receptor. My results, together with others in the lab and elsewhere, will help our understanding of how Myo2p is regulated, which is essential for the cell's survival.