The genetics of reproductive isolation in Leavenworthia

了解物种是如何形成的，是进化遗传学尚未回答的基本问题之一。 这对理解基因组进化是至关重要的。回答这个问题需要遗传基因的知识 物种间生殖隔离的基础。具体问题是：(1)什么是进化过程 造成物种间生殖障碍的原因是什么？(2)某些繁殖障碍出现的频率是多少？ 物种间的？；(3)与生殖隔离相关的数量性状有多少个基因座？ 物种之间？；以及(4)哪些基因控制物种之间的生殖隔离，以及它们是如何发挥作用的？至 开始回答这些问题，两个物种之间生殖隔离的生态学和遗传学基础 将确定莱文沃西亚属(L.alabamica和L.crassa)。互换移植实验 将决定是否存在生态地理隔离。将进行传粉者观察，看看是否 不同物种的传粉者偏好不同，这将是一种行为学上的孤立。莱文沃西亚 Alabamica和L.crassa在自然界中进行杂交，因此将检查杂交的生存能力，以确定是否有 受精卵后生殖障碍是存在的。将绘制数量性状座位图，以确定有多少遗传 基因座负责生殖隔离。这些QTL将与拟南芥的序列进行比较 作为已经消失的功能的线索。最后，在这些物种或他们的物种中观察到的任何近亲繁殖衰退的遗传学 混合动力车将被确定。

Understanding how species form is one of the fundamental unanswered questions of evolutionary genetics. And it is critical in understanding genome evolution. Answering this question requires knowledge of the genetic basis of reproductive isolation between species. Specific questions are: (1) what evolutionary processes are responsible for reproductive barriers between species?; (2) what are the frequency of certain barriers to reproduction between species'?.; (3) how many loci contribute to the quantitative traits associated with reproductive isolation between species?; and (4) what genes control reproductive isolation between species and how do they function? To begin to answer these questions, the ecological and genetic basis of reproductive isolation between two species in the plant genus Leavenworthia (L. alabamica and L. crassa) will be determined. Reciprocal transplant experiments will determine whether or not ecogeographic isolation is present. Pollinator observations will be conducted to see if pollinator preferences differ between species, which would be a form of ethological isolation. Leavenworthia alabamica and L. crassa hybridize in nature, and thus hybrid viability will be examined in order to determine if any post-zygotic barriers to reproduction exist. Quantitative trait loci will be mapped to determine how many genetic loci are responsible for reproductive isolation. These QTL will be compared to the sequence of Arabidopsis thaliana as a clue to gone function. Finally, the genetics of any inbreeding depression observed in these species or their hybrids will be determined.