Gene Library Resource for the Sea Urchin S. purpuratus

该提案寻求对紫海胆阵列基因库资源的支持， 紫球海胆这个资源是整个基因组材料的唯一提供者 除了为细胞和发育研究界提供服务外，它还致力于基因组测序工作。 资源有两个部分：一个机器人阵列库和一个计算设施。机器人 该设施生成，维护和分发排列的图书馆资料的研究communinity。 新的服务目标是网页，以提高系统的有用性。页面将详细说明：1） 干扰试剂、探针和特异性基因引物; 2）干扰蛋白的表达和功能; 海胆DNA序列与资源质粒克隆的连接; 4)实现特定软件和文档分发; 5）列出社区特定信息。 在研究部分，我们将提供cDNA文库，为基因组的注释提供信息。我们 计划：1）从成人桡神经产生组织特异性文库; 2）产生含有成人桡神经的文库， 3）构建包含成人口腔结构分化基因的文库; 4） 从青少年灯笼中产生肌肉基因库;以及5）确定现有成年灯笼的质量 组织文库 我们设想海胆基因组资源作为一个分布式系统的一部分， 组成了一个高度关联的可用的全基因组资源。我们作为一个门户， 实验发育生物学的经验，以生物信息学家。我们提出了生物信息学 工具和基因组序列收集数据库。所有的个人目标 以上是对这一观点的直接回应。

This proposal seeks support for the Arrayed Gene Library Resource for the purple sea urchin, Strongylocentrotus purpuratus. This Resource is the sole provider of genomic materials for the whole genome sequencing effort in addition to its service to the cell and developmental research community. There are two parts to the Resource: a robotic arrayed library and a computational facility. The robotics facility generates, maintains and distributes arrayed library materials to the research commuinity. The new service aims are web pages to enhance the usefulness of the system. The pages will detail: 1) perturbing reagents, probes, and specific gene primers used in this system; 2) expression and function of sea urchin transcription factors; 3) links between sea urchin DMA sequences and resource plasmid clones; 4) implement distribution of specific software and documentation; and 5) list community specific information. In the research component we will provide cDNA libraries to inform the annotation of the genome. We plan to: 1) to produce a tissue-specific library from adult radial nerve; 2) to produce a library containing adult skeletogenic genes; 3) to construct a library containing differentiation genes for adult oral structures; 4) to produce a library from the juvenile lantern for muscle genes; and 5) to determine the quality of existing adult tissue libraries We envision the Sea Urchin Genome Resource as part of a distributed system whose individual efforts make up a highly connected, useable whole genome resource. We act as a portal to bring the accumulated experience of experimental developmental biology to bioinformaticists. And we present the bioinformatics tools and databases of genomic sequence collections to the experimentalist. All of the individual goals detailed above are direct responses to this view.