Role of mRNA Decay in the Immune System

这个名为“信使核糖核酸衰变在免疫系统中的作用”的项目申请旨在 协调一直在研究转录后调控的研究人员的努力，特别是， 信使核糖核酸周转的机制。该项目将由西德尼·佩斯卡执导，他是 分子遗传学、微生物学和免疫学系和一名长期研究人员 对干扰素、细胞因子、信号转导和信使核糖核酸周转感兴趣。该计划项目包括 五个方案：1.黏附激活的单核细胞基因；2.调节CD154的ARE和Non-ARE通路 信使核糖核酸的稳定性；3.富含肿瘤坏死因子α的翻译调控；4.调控 5：调节T淋巴细胞RANKL的表达。 其中四人有研究mRNA寿命的经验；两名研究人员是 免疫学家。该计划项目包含一个行政核心和六个小核心，以使工作 效率高、效果好。核心是：A)管理设施；B)流式细胞仪设施；C)实时 聚合酶链式反应设备；C)成像设备；E)辐射设备；F)DNA合成和测序设备；G) 实时荧光共振能量装置。 这个应用程序的总体目标是了解mrna周转如何控制免疫的各个步骤。 免疫反应过程中的激活和分化。控制变化的快速方法之一 蛋白质的表达是通过改变mRNA的衰减率来实现的，这是一个转录后的过程。 了解信使核糖核酸在免疫调节中的作用 开发新的疗法来控制这些过程并治疗各种 以及其他免疫紊乱。

This Program Project application "The Role of mRNA Decay in the Immune System" is designed to coordinate the efforts of investigators who have been studying post-transcriptional regulation, in particular, the mechanisms involved in mRNA turnover. The program is to be directed by Sidney Pestka, chair of the Department of Molecular Genetics, Microbiology and Immunology and an investigator with a long term interest in interferons, cytokines, signal transduction and mRNA turnover. The Program Project consists of five projects: 1. Adherence Activated Monocyte Genes; 2. ARE & non-ARE Pathways Regulating CD154 mRNA Stability; 3. Translational Regulation by the TNF Alpha AU-rich Element; 4. Regulation of Cytokine Gene Expression by mRNA Turnover; 5: Regulation of RANKL Expression in T-Lymphocytes. Four of the individuals have experience in study of mRNA lifetime; two of the investigators are immunologists. The Program Project contains an administrative core and six small cores to make the work efficient and effective. The cores are: A) Administrative Facility; B) Flow Cytometry Facility; C) Real-Time PCR Facility; C) Imaging Facility; E) Irradiation Facility; F) DNA Synthesis & Sequencing Facility; G) Realtime Fluorescence Resonance Energy Facility. The overall aim of this application is to understand how mRNA turnover controls various steps in immune activation and differentiation during immune responses. One of the fast methods to control changes in protein expression is by modification of the rate ofmRNA decay, a post-transcriptional process. Understanding the role of mRNA turnover in regulation of the immune develop new therapeutics to control these processes and treat a variety and other immune disorders.