Mouse Genetics Core

本计划项目申请中提出的实验将大量使用转基因动物 已经在我们的实验室生产，或从其他研究人员，或从商业实验室获得。 总共提出了超过15个小鼠品系，包括转基因小鼠、敲入小鼠和敲除小鼠。 项目。 小鼠遗传学核心将维持每种动物繁殖所需的最低数量。 在每个项目中，根据PI的要求设置定时怀孕繁殖、断奶、幼犬ID(打耳机、 标记)、用于基因分型的组织收集、基因组DNA纯化、建立用于 基因分型，执行聚合酶链式反应分析，产生携带两个或更多个纯合子系或转基因系 转基因通过适当的杂交、记录保存、法援局与调查人员之间的联系 计划项目。 小鼠遗传学核心的意图是高效地产生足够数量的实验小鼠 对所有项目进行准确的基因分型。通过将这些活动集中起来，该核心将消除 通过为所有被研究的动物建立一个中央档案、存储和分发系统，努力和动物。 它还将高效利用设备，将提供容易获取的动物信息，标准化 作为调查人员和技术人员分配到核心的繁殖技术和套孕时间 在转基因小鼠和群体管理方面有丰富的经验。

Experiments proposed in this Program Project application will heavily use genetically altered animals already produced in our laboratories, or obtained from other investigators, or from commercial laboratories. More than 15 mouse lines, including transgenic, knock-in and knock-out mice, are proposed in the overall project. The mouse genetics core will maintain the minimum adequate numbers of animals for breeding for each ine, set timed pregnancy breeding as requested by the PI in each project, weaning, pup ID (ear punching, tagging), collection of tissue for genotyping, genomic DNA purification, establishing PCR conditions for genotyping, performing PCR analysis, generating homozygous lines or transgenic lines carrying two or more transgenes by adequate crossing, record keeping, link between the LASC and the Investigators in the Program Project. The intent of the mouse genetics core is to efficiently generate sufficient numbers of experimental mice accurately genotyped for all projects. By centralizing these activities this core will eliminate duplication of effort and animals by creating a central archive, storage and distribution system for all animals studied. Also it will make efficient use of equipment, will provide easy access to animal information, standardized breeding techniques and timing of set pregnancies as the investigators and technician assigned to the core have extensive experience in genetically modified mouse and colony management.