Cell Function Using NOD SCID Xenografts

一个共享的“鼠标核心”被提议作为程序项目的核心单元C。该核心的目标是 使研究者能够以及时和经济的方式进行体内研究。的责任 这个核心是双重的。首先，该核心将维持B6. SJL-PtrcaPep 3b/BoyJ（Boy.！） 小鼠和NOD.CB17-Pr/fc 1C-/J-非肥胖/严重联合免疫缺陷-（NOD/SCID）小鼠。单独 这些殖民地的繁殖和实验室位于邻近的医学科学中心， 在动物资源中心实验室的buildir g。BoyJ小鼠将用于各种 移植实验中，两个遗传上不同的来源的相对繁殖能力， 将研究严格的淘汰。此外，这些小鼠将用于研究亚清髓方案， 在体内选择低数量的转导干细胞的策略。NOD/SCID小鼠将用于 该项目的研究重点是人类干细胞和祖细胞生物学。第二，对于NOD/SCID 实验，核心领导将管理和协调与核心人员的各个方面， 移植此外，核心人员将进行NOD/SCID小鼠的移植，收获和 提供NOD/SCID实验的组织给本项目的研究者进行分析。这将 也可以与“细胞纯化和分析实验室（核心A）”协调。“核心领导人还将 提供实验设计和NOD/SCID干移植数据解释方面的帮助 移植实验共享核心A将为计划项目的参与者提供具有成本效益的 为他们的研究提供高质量的服务。这些研究将提供新的信息， 造血干细胞在体内的功能，适用于未来发展的基因治疗试验。

A shared "Mouse Core" is proposed as Core Unit C for the program project. The goal of this core is to enable the investigator to perform in vivo studies in a timely and economical manner. The responsibilities of this core are two-fold. First, this core will maintain breeding colonies for B6.SJL-PtrcaPep3b/BoyJ (Boy.!) mice and NOD.CB17-Pr/¿fciC"'/J-nonobese/severe combined immunodeficient-(NOD/SCID) mice. Separate breeding and experimental rooms for these colonies are conveniently located in the adjacent Medical Sciences buildir g in the Laboratory of Animal Resource Center. The BoyJ mice will be used in a variety of transplantation experiments in which the relative repopulating ability of two genetically distinct sources of stern culls will be studied. In addition these mice will be used to study submyeloablative regimens and strategies to select for low numbers of transduced stem cells in vivo. The NOD/SCID mice will be utilized in investigations focused on human stem and progenitor cell biology in this program. Second, for NOD/SCID experiments, the core leader will manage and coordinate with core personnel all aspects of the transplantation. In addition, core personnel will perform transplants into NOD/SCID mice, harvest and provide tissues from NOD/SCID experiments to investigators of this program project for analysis. This will also be coordinated with the "Cell Purification and Analysis Laboratory (Core A)." The core leader will also provide assistance with experimental design and interpretation of data generated in NOD/SCID xeriograft transplantation experiments. The shared Core A will provide participants in the program project with costeffective and high quality services for their research. These studies will provide new information regarding hematopoietic stem-cell function in vivo that is applicable to the future development of gene therapy trials.