Testing Surgery Guided by Molecular Fluorescence Imaging

描述（由申请人提供）： 该提案描述了一个为期5年的培训计划，为学术生涯的发展，在外科。主要研究者已完成医学博士/博士在华盛顿大学的耳鼻咽喉头颈外科住院医师培训项目和在加州圣地亚哥大学（UCSD）的ACGME认证的耳鼻咽喉头颈外科住院医师培训项目。她还完成了UCSD颅底外科的博士后研究，现在，她将通过跨部门专业知识和资源的独特整合，扩大她在临床医学和科学研究方面的综合培训。Roger Tsien博士将指导主要研究者的科学发展。钱博士是细胞生物学和神经生物学领域公认的领导者，并以开发用于监测细胞内过程的彩色荧光染料而闻名于世。自1989年以来，他一直是霍华德休斯研究员，是加州大学圣地亚哥分校药理学系的教授，并培养了许多博士后研究员和研究生。为了加强培训，该计划还将有一个由医生科学家组成的咨询委员会，他们是各自领域的领导者，将提供科学和职业建议。研究将集中于开发用于分子荧光引导手术的动物模型。具体目标包括：1）开发用于小动物荧光引导手术的成像系统，2）开发用于荧光引导手术的动物模型，具有切除准确性的定量客观标准3）开发用于全身成像与荧光图像引导手术结合的动物模型尽管最近在癌症的非侵入性诊断方面取得了技术进步，包括CT，MRI和PET成像，实体恶性肿瘤的主要治疗方式是手术切除。然而，肿瘤的手术切除仍然依赖于个体外科医生使用诸如肿瘤位置、纹理、颜色、与周围结构的关系等不可量化的标准来区分肿瘤与正常组织的能力。该项目的相关性是为手术外科医生提供可在真实的时间内可视化的敏感分子标记，以在手术中区分肿瘤与非肿瘤组织，从而引导手术切除。

DESCRIPTION (provided by applicant): This proposal describes a 5 year training program for the development of academic career in Surgery. The principal investigator has completed a combined M.D./Ph.D. program at the Washington University and an ACGME accredited residency training program in Otolaryngology-Head and Neck Surgery at the University of California San Diego (UCSD). She has also completed a postdoctoral fellowship in Skull Base Surgery at UCSD and now, will expand her combined training in clinical medicine and scientific research through an unique integration of interdepartmental expertise and resources. Dr. Roger Tsien will mentor the principal investigator's scientific development. Dr. Tsien is a recognized leader in the field of cell biology and neurobiology and is world renowned for developing colorful fluorescent dyes for monitoring intracellular processes. He has been a Howard Hughes Investigator since 1989, is a Professor in the Dept of Pharmacology at UCSD and has trained numerous postdoctoral fellows and graduate students. To enhance the training, the program will also have an advisory committee of physician scientists who are leaders in their fields and who will provide scientific and career advice. Research will focus on the development of an animal model for molecular fluorescence guided surgery. The specific aims include 1) develop an imaging system for fluorescence guided surgery in small animals, 2) develop animal models for fluorescence-guided surgery, with quantitative objective criteria for accuracy of excision 3) develop animal models for whole body imaging coupled with fluorescence image guided surgery Despite recent technological advances in the noninvasive diagnosis of cancers including CT, MRI and PET imaging, the main treatment modality for solid malignant tumors is surgical excision. However, surgical excision of tumors remains dependent on the individual surgeon's ability to differentiate tumor from normal tissue using non-quantifiable criteria such as tumor location, texture, color, relationship to surrounding structures etc. The relevance of this project is to provide the operating surgeon with a sensitive molecular marker visualizable in real time to differentiate tumors from non-tumor tissue intraoperatively, thereby guiding surgical excision.