Circular Dichroism Spectrometer

描述（由申请人提供）：本项目的目标是购买一个国家的最先进的高灵敏度圆二色光谱仪（JASCO J-815），并使其提供给整个阿尔伯特爱因斯坦医学院的科学界。该仪器将取代使用了18年的CD光谱仪，后者灵敏度低得多，可靠性差，功能有限。新的JASCO仪器的能力将远远超出校园内目前可用的任何仪器，因为它将结合联合收割机圆二色性，光学吸收和荧光光谱，可以在平衡，滴定，温度控制和停流条件下进行。在美国国立卫生研究院的支持下，有几个用户对仪器有基本需求，以实现其项目的目标。一旦进入校园，预计科学界的许多其他成员也将利用这种仪器来开展他们的项目。将通过电子邮件宣布该仪器的可用性，并将在一个新的网站上永久提供关于其能力的全面说明沿着时间表信息和技术文件。 计划中的仪器项目涵盖了广泛的生物医学领域，包括生理学上重要酶的几种酶促过程的机理研究，被认为与人类病原体中的药物和/或宿主抗性有关的蛋白质的研究，M。结核和C. jejuni，血液代用品的表征，其在临床试验中，过渡态抑制剂的设计和表征，其在白血病T细胞自身免疫性疾病的临床试验中，以及1-突触核蛋白中纤维形成过程的测定，1-突触核蛋白是帕金森病中神经缠结聚集体的主要成分。 这一工具将在近期和长期内对公共卫生非常重要。它将立即在血液替代品和抗癌药物的开发方面产生更好的理解和进步。从长远来看，它将提供有关人类病原体关键蛋白质的新信息，这可能会允许新的抗微生物策略，最后，有关几种人类酶的新机制信息将对未来的临床研究有价值，包括了解疾病和开发新的药物制剂。

DESCRIPTION (provided by applicant): The goal of this project is to purchase a state-of-the-art high sensitivity Circular Dichroism Spectrometer (JASCO J-815) and to make it available to the entire Albert Einstein College of Medicine scientific community. This instrument will replace an 18-year old CD spectrometer that has much lower sensitivity, poor reliability and limited functions. The capabilities of the new JASCO instrument will extend well beyond those of any instrument currently available on the campus, as it will combine circular dichroism, optical absorption and fluorescence spectroscopies that can be made under equilibrium, titration, temperature-controlled and stopped-flow conditions. There are several users, with National Institutes of Health support, who have an essential need of the instrumentation to fulfill the aims of their projects. Once on campus it is anticipated that many other members of the scientific community will take advantage of this instrumentation to carry out their projects as well. The availability of the instrument will be announced by e-mail and a full description of its capabilities, along with scheduling information and technical documents, will be permanently available on a new web site. The planned projects with the instrumentation span a wide spectrum of biomedical areas including mechanistic studies of several enzymatic processes in physiologically important enzymes, studies of proteins that are believed to be involved in conferring drug and/or host resistance in the human pathogens, M. tuberculosis and C. jejuni, characterization of blood substitutes, which are in clinical trials, design and characterization of transition state inhibitors, which are in clinical trials for leukemia T-cell autoimmune disease, and determination of the fiber formation processes in 1-synuclein, the principle component of the neural tangle aggregates in Parkinson's disease. This instrumentation will be very relevant to public health in both the near and long term. It will immediately yield better understanding and advances in the development of blood substitutes and anti-cancer drugs. In the longer term, it will give new information on critical proteins from human pathogens, which may allow for new anti-microbial strategies, and finally, new mechanistic information on several human enzymes will be valuable for future clinical studies including the understanding of diseases and the development of new pharmaceutical agents.