Integrated, Modular Equipment for Large-scale and Small-scale Peptide Synthesis

描述（由申请人提供）：所要求的仪器将装备加州大学洛杉矶分校David Geffen医学院的肽核心实验室，该实验室是美国首屈一指的生物医学研究机构之一。它将包括一个用于中小规模多肽生产的多肽合成器，一个带集成在线质谱系统的制备型高效液相色谱装置，冷冻干燥装置和一个化学安全罩。该设施生产的肽将用于研究：a) hepcidin，一种最近发现的23残基肽，是人类铁摄取和利用的主要调节剂；b)反转录细胞周期素，预防HIV-1、流感和疱疹病毒感染的环状八肽；c) β -淀粉样蛋白，一种与阿尔茨海默病发病机制有关的肽；d)抗氧化肽，如D4F，具有降低动脉粥样硬化风险的潜力；E)免疫调节肽，最终可以改善自身免疫性疾病（如狼疮）患者的治疗；F)阻断受体介导的炭疽毒素进入和损伤细胞的能力的肽；g)可以功能化新型纳米复合材料（“量子点”）的肽，并促进其作为新型成像和药物递送剂的发展。

DESCRIPTION (provided by applicant): The instrumentation requested will equip a Peptide Core Laboratory at the David Geffen School of Medicine at UCLA, one of the nation's premier biomedical research institutions. It will consist of a multiple peptide synthesizer for small and intermediate scale peptide production, a preparative HPLC unit with integrated on- line mass spec system, freeze drying apparatus, and a chemical safety hood. Peptides produced in this facility would be used to study: a) hepcidin, a recently discovered 23-residue peptide that is a master regulator of iron uptake and utilization in humans; b) retrocyclins, cyclic octadecapeptides that prevent infection by HIV-1, influenza and herpes viruses; c) beta-amyloid, a peptide whose properties are involved in the pathogenesis of Alzheimer's Disease; d) antioxidant peptides, such as D4F, with the potential to decrease the risk of atherosclerosis; e) immunoregulatory peptides, that could eventually improve the treatment of patients with autoimmune disorders, such as lupus; f) peptides that can block the receptor-mediated ability of anthrax toxins to enter and damage cells; and g) peptides that can functionalize novel nanocomposite materials ("quantum dots"), and promote their development as novel imaging and drug-delivery agents. Each of these projects depends on peptides that are not available "off-the-shelf". Consequently, the studies are often impeded by the expense or difficulty of obtaining peptides that may be cyclic (retrocyclins, D4F), or have multiple disulfide bonds (retrocyclins and hepcidin).