Hypersense DNP System

描述（由申请人提供）：本申请用于购买Hypersense动态核偏振器（DNP）仪器，该仪器已经借出，并位于高级成像研究中心，靠近窄孔600 MHz NMR光谱仪。该中心研究人员的一个共同目标是使用13 C同位素分析研究代谢通量。同位素分析方法的不同体现已被用于研究灌注器官和细胞以及小鼠，大鼠和人类。各种代谢途径也已被测定，包括克雷白氏循环，糖原分解，β-氧化，生酮等。通常，研究是在从受试者抽取的血液提取物上进行的。 所有这些应用的共同点是需要提高13 C NMR研究的灵敏度。目前，两个14.1特斯拉的磁铁致力于在UT西南代谢研究。即使有出色的硬件可用，小鼠和大鼠的生存实验是不可行的，因为在合理的时间内获得13 C光谱所需的代谢物的量。在大多数小鼠研究中，必须将两到三只动物的血液合并以分离单个代谢物用于NMR分析。随着Hypersense技术的加入，小鼠的生存实验至少应该可以实现。Hypersense有可能使NMR的灵敏度提高10000倍，从而改变同位素分析的应用范围。也许更重要的是，Hypersense已经证明了在灌注器官中阐明代谢通量的能力。已经在大鼠心脏中进行了碳酸氢盐产生和克雷布循环周转的真实的时间测量。 超义DNP是NMR实验灵敏度的根本变化。将这项技术整合到已经在进行的研究中，有望使13 C同位素分析的能力发生质的飞跃。

DESCRIPTION (provided by applicant): This application is for the purchase of a Hypersense Dynamic Nuclear Polarizer (DNP) instrument which is already on loan and sited in the Advanced Imaging Research Center in close proximity to a narrow bore 600MHz NMR spectrometer. A common goal of the investigators in the center is the study of metabolic fluxes using 13C isotopomer analysis. Different incarnations of the isotopomer analysis method have been used to study perfused organs and cells as well as mice, rats, and humans. A variety of metabolic pathways have been assayed as well, including the Kreb's cycle, gluconeogenesis and glycogenolysis, b-oxidation, ketogenesis, etc. Often, the studies are carried out on extracts of blood drawn from the subject. Common to all these applications is a need for enhanced sensitivity for 13C NMR studies. Currently, two 14.1Tesla magnets are dedicated to metabolic studies at UT Southwestern. Even with the outstanding hardware available, survival experiments on mice and rats are not feasible due to the quantity of metabolite needed to acquire the 13C spectra in a reasonable amount of time. In most mouse studies, blood from two to three animals must be pooled to isolate single metabolites for NMR analysis. With the addition of the Hypersense technology, survival experiments for mice should be attainable at the very least. Hypersense has the potential for 10000 time gains in sensitivity for NMR, transforming the range of applications for isotopomer analysis. Perhaps even more importantly, the Hypersense has already demonstrated the ability to elucidate metabolic flux in perfused organs. Real time measurements of bicarbonate production and Kreb's cycle turnover have already been made in rat hearts. Hypersense DNP is a fundamental change in the sensitivity for NMR experiments. Integration of this technology into research already underway promises a quantum leap in the power of 13C isotopomer analysis.