BAC Library Production and Maintenance

目标是为物种准备10倍冗余的细菌人工染色体(BAG)文库 由美国国立卫生研究院咨询委员会挑选，并根据国家卫生研究院的政策传播图书馆。这个 图书馆将根据科学界对比较基因组学的兴趣而量身定做，并用于 支持全基因组测序项目。这个项目是早先一个合作项目的延续 美国国立卫生研究院和奥克兰儿童医院研究所之间的协议。该应用程序具有 与准备(BAC)相关的四个组成部分：1)库准备，2)克隆阵列和库 复制，3)表征，4)研究和开发，以提高工艺效率和袋子 图书馆质量。在第一年，将为具有典型的 哺乳动物的基因组大小。这相当于80万袋克隆的准备能力。在这些年里 第二和第三项计划，改进袋子施工方法可能会导致略有增加 吞吐量。延续计划的规模太小，不能提供“规模经济”的机会。 改进。新的图书馆将补充已有的大量袋子克隆收藏曲目 可用于比较基因组分析。新的克隆集合将通过标准化的集合进行分析 一系列测试，包括使用一组基因组标记进行筛选、有限的BAC末端测序和插入大小 决心。一旦图书馆通过质量控制，新的资源将在一个 格式与主要应用程序一致。为了方便人们进入袋子图书馆，一个基于 关于按使用费收回成本的问题已经确立。预计新的袋子资源将继续 通过比较人类基因有助于更好地了解人类基因的功能和进化 随着动物种类的增加，基因的种类也越来越多。袋子克隆也将越来越多地被用作 建立人类疾病的动物模型。克隆人的广泛传播是必不可少的 项目的组件。大多数图书馆的使用费不足以支付维护费用。因此， 要求提供最低限度的经费，以支持维护新的图书馆和在 之前的计划倡议。有关可通过bacpac网站访问的图书馆的信息 资源(http://bacpac.chori.org).

The goal is to prepare tenfold redundant Bacterial Artificial Chromosomes (BAG) libraries for species selected by an NIH advisory committee and to disseminate the libraries in accordance with NIH policy. The libraries will be tailored to the scientific community's interest in comparative genomics and be used in support of whole-genome sequencing projects. The project is a continuation of an earlier cooperative agreement between the NIH and the Children's Hospital Oakland Research Institute. The application has four components relevant to preparing (BACs): 1) Library preparation, 2) Clone Arraying & Library Duplication, 3) Characterization, and 4) Research & Development to improve the process efficiency and BAG library quality. During the first year, four BAG libraries will be prepared for genomes having a typical mammalian genome size. This corresponds with an "800,000 BAG clone" preparation capacity. During years two and three of the program, improvements in BAG construction methods may result in slight increases in throughput. The small size of the continuation program does not offer opportunities for "economy of scale" improvements. The new libraries will complement the extensive repertoire of BAG clone collections already available for comparative genome analysis. The new clone collections will be analyzed by a standardized set of tests, including screening with a set of genomic markers, limited BAC-end sequencing and insert size determination. Once the libraries pass the quality controls, the new resources will be made available in a format consistent with major applications. To facilitate access to the BAG libraries, a screening service based on cost recovery by user fees has been established. It is expected that the new BAG resources will continue to contribute to better understanding of human gene function and evolution through comparison of human genes with a growing spectrum of animal species. BAG clones will also increasingly be used as tools to create animal models for human diseases. Widespread dissemination of the clones is an essential component of the project. User fees for most libraries cannot cover the costs of maintenance. Hence, mimimal funding is requested in support of the maintenance of the new libraries and those generated under the previous program initiative. Information about libraries available through the website for BACPAC Resources (http://bacpac.chori.org).