CORE--HISTOLOGY

Core C是资源核心。这三个项目都将广泛依赖于小鼠的组织学分析， 心中设备、技术专门知识和试剂的集中将促进这些活动， 最大限度地减少相关成本。核心C的具体活动包括（1）协助常规石蜡 包埋和切片，（2）辅助常规冷冻保存和切片，（3）辅助 标准组织化学染色（即，苏木精和伊红，阿尔辛蓝，马森三色，天狼星红， fast绿色，von Kossa等），（4）协助原位杂交，和（5）协助常规免疫 组织学分析（即，TUNEL、BrdU、肌节肌动蛋白、肌球蛋白重链等）。鉴于沉重的 由于所有三个项目都利用了组织学分析，核心C对成功完成 提出的实验。

Core C is the Resources Core. All three Projectswill rely extensively on histological analysis of mouse hearts. Centralization of equipment, technical expertise and reagents will facilitate these activities and minimize the associated costs. Specific activities of Core C include (1) assistance with routine paraffin embedding and sectioning, (2) assistance with routine cryopreservation and sectioning, (3) assistance with standard histochemical staining (i.e., Hematoxylin and Eosin, Alcian blue, Masson's trichrome, Sirius red and fast green, von Kossa, etc), (4) assistance with in situ hybridizations, and (5) assistance with routine immune histology analyses (i.e., TUNEL, BrdU, sarcomeric actin, myosin heavy chain, etc). Given the heavy utilization of histologic analyses by all three projects, Core C is essential for the successful completion of the proposed experiments.