Cell and Tissue Kinetics Core

开发细胞和组织动力学核心是为了通过协助该中心促进与皮肤相关的研究 在二维和三维培养中分离和培养原代皮肤细胞的研究人员 高效系统构建转基因组织模型分析皮肤病相关基因 表现出转基因过度表达或抑制的皮肤。具体目标如下： 1.在分离和培养小鼠和人类皮肤细胞方面提供援助和培训，包括 角质形成细胞、真皮成纤维细胞、黑素细胞，并为三维构筑提供专业知识 从正常细胞、疾病细胞或转基因细胞中培养出人类皮肤。 2.为角质形成细胞干细胞的各种体外功能检测提供帮助和培训。 3.提供并开发合适的转基因皮肤模型以研究正常或正常皮肤的功能后果 突变基因在人和小鼠表皮中的表达。 皮肤常规形态分析后会给细胞和组织动力学核心带来问题 核心或细胞培养的表型显示了一个不同寻常的发现。细胞和组织核心的发现 如果它们提示基因表达或其他方面的特定变化，将被称为分子生物学核心 分子生物学方面的问题已经出现。细胞和组织动力学核心致力于提供 为中心调查人员提供服务和培训，以发展进行这些实验所需的技能 在他们自己的实验室里。该核心提供的服务将不仅有利于试点和可行性 这项研究包括在此申请表中，但适用于#年可能被皮肤病研究中心招募的研究 未来。

The Cell and Tissue Kinetics Core was developed to facilitate skin-related research by assisting the center investigators in isolation and cultivation of primary skin cells in two and three-dimensional cultures and in analysis of genes related to skin diseases by efficient and systematic generation of transgenic tissue models of skin that show either transgene overexpression or suppression. The Specific Aims are as follows: 1. To provide assistance and training in isolation and culture of mouse and human skin cells including keratinocytes, dermal fibroblasts, melanocytes and to provide expertise in construction of three dimensional cultures of human skin from normal cells, diseased cells or transgenic cells. 2. To provide assistance and training in a variety of functional in vitro assays for keratinocyte stem cells. 3. To provide and develop proper transgenic skin models to study functional consequence of normal or mutant gene expression in human and mouse epidermis. Problems will be brought to the Cell and Tissue Kinetics Core after routine morphological analysis in the Skin Phenotyping Core or cell culture revealed an unusual finding. Findings made by the Cell and Tissue Core will be referred to the Molecular biology Core if they suggest specific changes in gene expression or other problems in molecular biology have occurred. The Cell and Tissue Kinetics Core is committed to provide both services and training for center investigators to develop necessary skills to perform these experiments in their own laboratories. The services provided by this core will benefit not only the pilot and feasibility studies included in this application but to those likely to be recruited to the Skin Diseases Research Center in the future.