Physiology Core

Physiology Core支持全面的、最先进的骨生理表征 和其他肌肉骨骼组织。核心由3个“子单位”组成， 主任，托马斯卡彭特博士。核心的服务订阅量非常大，它是最大的 三大科学核心。 组织学和组织形态学亚单位，由Friedlaender博士和 提供高质量的骨骼组织的组织学和组织形态学分析。最近 这些举措包括原位TUNEL检测的发展，以及新的免疫组织化学 根据特定调查人员的需要进行染色。最近由Dr. 大卫克，一个全国公认的啮齿动物组织形态学专家，得出结论， 提供的都是极高质量的。 博士Caren Gundberg是该领域国际公认的专家， 骨转换亚单位的标志物。该亚单位提供骨转换标记物和血清的测定 PTH。矿化骨中骨钙素、钙、磷酸盐和碱性磷酸酶的测定 细胞培养是实验室的新举措。 骨密度测定亚单位支持通过外周定量骨密度测量 计算机断层扫描（pQCT）和双能吸收测定法（PlXlmus）通过协议 细胞核心 核心的具体目标是：1）继续向中心提供上述概述的服务 以协调和有效的方式，从而扩大其范围和生产力， 工作; 2）为中心成员提供这些技术的培训; 3）继续发展和 评估与体内和体外骨骼组织分析相关的新方法。

The Physiology Core supports comprehensive, state-of-the-art physiological characterization of bone and other musculoskeletal tissues. The Core is comprised of 3 "subunits" which are coordinated by the director, Dr. Thomas Carpenter. The services of the Core are very heavily subscribed, and it is the largest of the three scientific cores. The Histology and Histomorphometry Subunit, which is co-supervised by Drs. Friedlaender and Carpenter, provides high quality histologic and histomorphometric analyses of skeletal tissue. Recent initiatives have included the development of an in situ TUNEL assay, and new immunohistochemical stains in response to the needs of specific investigators. A recent external audit of the subuint by Dr. David Ke, a nationally recognized expert in rodent histomorphometry, concluded that the services being provided are of extremely high quality. Dr. Caren Gundberg, an internationally recognized expert in the field, supervises the Biochemical Markers of Bone Turnover Subunit. This subunit offers assays of bone turnover makers and serum PTH. Assays for media osteocalcin, calcium, phosphate, and alkaline phosphatase, in mineralizing bone cell cultures are new initiatives in the laboratory. The Bone Densitometry Subunit supports measurement of bone density by peripheral quantitative computerized tomography (pQCT) and by dual energy absorptiometry (PlXlmus) through an agreement with the Cell Core. The specific aims of the Core are to: 1) continue to provide the above summarized services to Center members in a coordinated and efficient manner, thereby extending the range and productivity of their work; 2) to offer training in these techniques to Center members and 3) to continue to develop and evaluate new methodologies relevant to the analysis of skeletal tissue in vivo and in vitro.