Proteomic Biomarkers for Primary Open Angle Glaucoma

描述（由申请人提供）：原发性开角型青光眼（POAG）是全球老年人致盲的主要原因之一。减缓或预防该病的进展是一项紧迫的公共卫生目标。我们假设易患POAG的个体的血液中存在生物标记物，这些生物标记物可以在疾病的临床证据出现之前识别出那些有风险的人。提出了一项病例对照试验研究，以验证血浆中含有POAG诊断生物标志物的假设。血浆将从美国临床记录的患者中收集，分为四个研究组，包括一个对照组和三个POAG组（高眼压、可能的POAG和明确的POAG）。ELISA将用于筛选血浆中可能作为POAG血浆生物标志物的蛋白质氧化修饰。高分辨率MALDI TOF和QTOF质谱将用于筛选血浆蛋白质组学模式和/或确定POAG诊断血浆肽的序列。长期目标是发展用于临床医学的常规诊断技术，以预测POAG的易感性和监测治疗效果，基本上就像今天用于心血管疾病风险管理的血清胆固醇测量一样。本研究将为早期发现“POAG高危”个体提供新方法，为测量POAG治疗效果提供新方法，并为青光眼病理机制提供新见解。公共卫生相关性：原发性开角型青光眼（POAG）是最常见的青光眼，影响着约300万美国人和全球超过7000万人。减缓或预防该病的进展是一项紧迫的公共卫生目标。本研究将为早期发现POAG高危人群提供新方法，为测量POAG治疗效果提供新方法，并为青光眼病理机制提供新见解。

DESCRIPTION (provided by applicant): Primary open angle glaucoma (POAG) is one of the leading causes of blindness in the elderly population worldwide. Slowing or preventing the progression of this disease is an urgent public health goal. We hypothesize that biomarkers exist in the blood of individuals susceptible to developing POAG that will allow identification of those at risk prior to clinical evidence of the disease. A case-control pilot study is proposed to test the hypothesis that plasma contains diagnostic biomarkers for POAG. Plasma will be collected from clinically documented patients from the United States in four study groups, including a control group and three POAG groups (ocular hypertension, possible POAG and definite POAG). ELISA will be used to screen plasma for protein oxidative modifications that may serve as POAG plasma biomarkers. High resolution MALDI TOF TOF and QTOF mass spectrometry will be used to screen plasma for proteomic patterns and/or to determine the sequence of POAG diagnostic plasma peptides. The long term goal is the development of routine diagnostic technology for use in clinical medicine to predict POAG susceptibility and to monitor therapeutic efficacy, essentially as serum cholesterol measurements are used today for risk management for cardiovascular disease. This research will lead to methods for early detection of "POAG at risk" individuals, new methods for measuring POAG therapeutic efficacies and new insights into mechanisms of glaucoma pathology. PUBLIC HEALTH RELEVANCE: Primary open angle glaucoma (POAG) is the most common form of glaucoma and affects about 3 million Americans and over 70 million people worldwide. Slowing or preventing the progression of this disease is an urgent public health goal. The proposed research will lead to methods for early detection of "POAG at risk" individuals, new methods for measuring POAG therapeutic efficacies and new insights into the mechanisms of glaucoma pathology.