CORE--HISTOCHEMISTRY

为了加快作为Van Eldik、LaDu和Vassar项目组成部分的组织化学和细胞化学调查，将建立一个核心设施，提供组织学服务、培训和专业知识。核心设施将配备两名技术人员、一名主任和一名联席主任，并将配备用于对小鼠大脑切片进行切片和染色，然后可能会通过附近的细胞成像设施的许多技术进行检查。这个核心将提供三种主要类型的服务：1)我们将使用标准化的抗体和组织试剂小组对各个项目提供的小鼠大脑进行切片和染色，这些抗体和组织试剂包括激活标记和神经退化标记。2)我们将对项目人员进行培训，使其掌握适合其项目形态方面的程序。这将包括光学显微镜、共焦显微镜和电子显微镜以及相关的图像捕获和形态测量程序。这也将包括固定和染色方案，用于他们对培养细胞的实验。3)我们将在个别项目的组织学部分的设计、执行和解释方面提供我们的专业知识。

In order to expedite the histochemical and cytochemical investigations that are integral to Projects by Van Eldik, LaDu and Vassar, will establish a core facility to provide histological services, training, and expertise. The core facility will be staffed by two technicians, a director, and a co-director and will be equipped for the sectioning and staining of mouse brain sections, which may then be examined by a number of techniques in the nearby Cell Imaging Facility. Three major types of service will be provided by this Core: 1) We will section and stain mouse brains provided by the individual projects, using a standardized panel of antibodies and histological reagents that includes markers of activation and markers of neurodegeneration. 2) We will train Project personnel in procedures appropriate to the orphological aspects of their Projects. This will include light, confocal, and electromicroscopical microscopy and relevant image-capture and morphometric procedures. This will also include fixation and staining protocols for use in their experiments with cultured cells. 3) We will make available our expertise in the design, execution, and interpretation of the histological portions of the individual Projects.