CORE--PROTEOMICS RESOURCE

该核心将为这个拟议的多囊肾病（PKD）研究小组的研究人员提供蛋白质组学技术和支持。虽然特定的基因和由基因编码的蛋白质中的突变已被确定为ARPKD和ADPKD的起点，但突变影响多种组织，因此几乎可以肯定的是除了由突变基因编码的主要蛋白质之外的多种蛋白质。蛋白质组学技术允许分析主要遗传缺陷下游的蛋白质变化模式;我们将利用现有DAB综合癌症中心（CCC）蛋白质组学/质谱共享设施的资源和人员，提供蛋白质组学和质谱分析。 对PKD研究工作的能力。这些努力将由现有的共享设施董事海伦·金博士和斯蒂芬·巴恩斯博士指导，他们为UAB生物医学研究界提供此类服务方面积累了丰富的经验。该核心的基本原理是，对来自假设驱动的PKD实验的生物样本进行系统的蛋白质组学分析，将提高识别参与PKD表型发病机制的先前未识别的蛋白质或蛋白质修饰的可能性。然后，这些结果可以识别用于后续研究的蛋白质，或者可以为PKD社区提供抗体。该中心的一项重要职能是为以下方面提供教育支持： 在蛋白质组学技术方面，为PKD社区提供支持，并使研究人员能够知情地利用核心技术，特别是对于那些以前没有接触过这些技术的人。这些活动将以辅导和/或讲习班的形式进行，以及由核心主任和研究人员个人参加的咨询会议。通过让生物统计学家与核心Meleth博士密切合作，PKD核心服务将得到加强;强烈鼓励PKD研究者在进行实验前咨询Meleth博士，以确保解决各种质量控制和实验设计问题。最后，一名研究助理将专门负责该核心，他将通过适当的2D分离和MS蛋白质鉴定来处理PKD样品。她将协调生成每组样本的最终数据报告。这一核心也将保持 PKD蛋白质组学数据库，作为共享设施正在进行的努力的一部分。

This Core will provide proteomics technology and support to the investigators in this proposed polycystic kidney disease (PKD) research group. While specific genes and mutations in the proteins encoded by the genes have been identified as starting points for ARPKD and ADPKD, the mutations affect multiple tissues, and therefore almost certainly multiple proteins other than the primary ones encoded by the mutated gene. Proteomics technology allows analysis of patterns of protein changes downstream of a primary genetic defect; we will utilize the resources and personnel of the existing DAB Comprehensive Cancer Center (CCC) Proteomics/Mass Spectrometry Shared Facility to provide proteomics and mass spectrometry capabilities toward the PKD research effort. These efforts will be directed by the existing Shared Facility directors, Drs. Helen Kim and Stephen Barnes, who bring substantial experience in providing such services to the UAB biomedical research community. The rationale for this Core is that systematic proteomic analysis of biological samples from PKD experiments that are hypothesis-driven will enhance the likelihood of identifying previously unidentified proteins or protein modifications that involved in the pathogenesis of the PKD phenotype. Such results can then identify proteins for followup studies, or to which antibodies can be raised for use by the PKD community. An important function of this Core is to provide educational support to the PKD community regarding proteomics technologies and to enable informed utilization of the core technologies by the investigators, particularly for those who have not previously accessed the technologies. These will be in the form of tutorials and/or a workshop, as well as consultation sessions involving the Core directors and individual researchers. PKD this Core services will be enhanced by having a biostatistician working closely with the Core, Dr. Meleth; PKD investigators will be strongly encouraged to consult with Dr.Meleth prior to carrying out an experiment, to insure that various quality control and experimental design issues are addressed. Finally, a research associate will be dedicated to this Core, who will process PKD samples through the appropriate 2D separations and the MS protein identifications. She will coordinate the generation of reports of the final data for each set of samples. This Core will also maintain a database of PKD proteomics data, as part of an ongoing effort by the Shared Facility.