Immunology Core

在约翰·D博士的指导下，奥特曼，核心H的使命，埃默里CFAR免疫学核心 实验室（ICL），是为埃默里社区提供最高质量的评估免疫学 研究免疫缺陷病毒的发病机制、治疗和预防所必需的功能 人类和非人类灵长类动物的感染。ICL将通过以下方式实现这一使命 具体目标： 1.开发和执行优化的、标准化的和经验证的细胞免疫功能测定， 接种疫苗的受试者、HIV感染的人和SIV感染的非人灵长类动物（NHP）; 2.创建来自Yerkes实验室的未处理（未研究）猴的冷冻保存PBMC库 灵长类动物研究中心，从而为CFAR研究人员提供了获得基本阴性对照的途径 用于试验开发和验证的样品。 3.通过提供共享的免疫试剂资源实现规模经济， 多个独立实验室; 4.维护CFAR流式细胞仪核心，以提供Emory CFAR成员，以及 更大的艾滋病研究社区，拥有最高质量的流式细胞术数据和细胞分选 提供的服务，包括处理生物危害性样品; 5.促进CFAR调查人员的免疫测定教育和培训机会， 亚特兰大艾滋病研究社区，以及国家和国际艾滋病调查人员。 通过这些目标，ICL将使所有埃默里CFAR调查人员能够利用创新和 强大的现代方法来定量分析先天性和后天性体液和细胞介导的 埃默里大学的研究人员和其他地方的科学家已经开发出了免疫力。准确 细胞介导的免疫应答的表征对于指导临床前疫苗开发是必不可少的， 用于在临床前和临床试验中确定疫苗效力的可能相关性， 了解艾滋病的发病机制。

Under the direction of Dr. John D. Altman, the mission of Core H, the Emory CFAR Immunology Core Laboratory (ICL), is to provide the Emory community with the highest quality assessments of immunological function necessary for the study of the pathogenesis, treatment, and prevention of immunodeficiency virus infections in humans and non-human primates. The ICL will achieve this mission through the following Specific Aims: 1. Develop and perform optimized, standardized, and validated assays of cellular immune function in vaccinated subjects, in HIV-infected humans, and in SIV-infected non-human primates (NHP); 2. Create a repository of cryopreserved PBMC from naive (off-study) monkeys from the Yerkes Primate Research Center, thereby providing CFAR investigators with access to esse'ntial negative control samples for assay development and validation. 3. Achieve economies of scale through the provision of immunological reagent resources shared by multiple independent laboratories; 4. Maintain a CFAR Flow Cytometry Core, for the purpose of providing members of the Emory CFAR, and the larger AIDS research community, with the highest quality flow cytometry data and cell sorting services available, including handling of biohazardous samples; 5. Promote immunological assay education and training opportunities for CFAR investigators, the AIDS research community in Atlanta, and national and international AIDS investigators. Through these Aims, the ICL will enable all Emory CFAR investigators to take advantage of innovative and powerful modern approaches to quantitative analysis of innate and acquired humoral and cell-mediated immunity that have been developed by individual Emory investigators and scientists elsewhere. Accurate characterization of cell-mediated immune responses is essential for guiding preclinical vaccine development, for determining possible correlates of vaccine efficacy in preclinical and clinical trials, and for better understanding AIDS pathogenesis.