Skin Cell Culture

描述（由申请人提供）：皮肤细胞培养核心（SCCC）旨在促进 UAB校园范围内的皮肤病研究中心（SDRC）成员之间的皮肤细胞生物学的跨学科研究。SCCC将提供从小鼠或人类皮肤中产生和维持表皮和真皮细胞特异性培养物的专业知识。此外，它还将提供RAFT皮肤等效培养物开发方面的专业知识。核心的具体目标是：1）以有效的成本效益方式为中心成员提供表皮和真皮细胞类型的中心来源，2）保持质量控制措施以确保无污染培养，3）提供专门的细胞培养系统和人和小鼠细胞转染方面的专业知识，以及4）开发用于产生混合共-培养具有朗格汉斯细胞和/或黑素细胞的筏角质形成细胞，以预期SDRC成员的兴趣和需求增加。

DESCRIPTION (provided by applicant): The Skin Cell Culture Core (SCCC) aims to promote interdisciplinary research in skin cell biology among UAB campus-wide Skin Diseases Research Center (SDRC) members. The SCCC will provide expertise in the generation and maintenance of epidermal and dermal cell specific cultures from either mouse or human skin. In addition, it will provide expertise in the development of RAFT skin equivalent cultures. The specific aims of the core are l) to provide a central source of epidermal and dermal cell types to Center members in an efficient cost effective manner, 2) to maintain quality control measures to ensure contamination-free cultures, 3) to provide expertise in specialized cell culture systems and transfection for both human and mouse cells and 4) to develop methods for generating mixed co-cultures of raft keratinocytes with Langerhans cells and/or melanocytes to anticipate increased interest and demand by SDRC members.