NEOANTIGENICITY OF RIBOFLAVIN AND LIGHT TREATED RBCS IN BABOONS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本研究旨在探讨用核黄素和紫外光进行Mirasol病原体减数技术(MPRT)处理的红细胞是否会产生免疫应答。第二个目标是评估经MPRT治疗的狒狒红细胞的存活和恢复情况。将未经处理或经米拉索处理的自体红细胞与明胶佐剂配制，并注射到各自的狒狒捐赠者体内。在未经处理或经MPRT处理的狒狒中，均未观察到对红细胞的抗体反应。接下来，用CR51标记未经处理和MPRT处理的红细胞，并将其回输给捐赠者。在未经处理和MPRT处理的狒狒中，存活和恢复动力学非常相似。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study was conducted to determine whether an immune response would be elicited to RBCs treated with the Mirasol pathogen reduction technology (MPRT) using Riboflavin and UV light. The second objective was to assess the survival and recovery of MPRT treated RBCs in baboons. Autologous RBC's either untreated or Mirasol-treated were formulated with alum adjuvant and was injected into the respective baboon donors. No antibody response to RBCs was observed either in the untreated or MPRT treated baboons. Next, untreated and MPRT treated RBCs were labeled with CR51 and infused back into the donors. Survival and recovery kinetics were quite similar in the untreated and MPRT treated baboons.