VIRULENCE GENE REGULATION IN STAPHYLOCOCCUS AND IDENTIFICATION OF SMALL MOLECUL

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 金黄色葡萄球菌是主要的动物病原体之一，由于缺乏有效的疫苗以及在社区相关或医院相关中出现多种耐药菌株，其仍然是主要的公共卫生问题。 葡萄球菌致病是一个复杂的多因素过程，涉及多个调控系统。调控系统的功能或基本生物过程的转录调控是基于上游启动子区域的DNA-蛋白质和蛋白质-蛋白质相互作用;因此，了解参与基因调控的网络的作用将有助于开发新的药物来控制和预防葡萄球菌感染。这些调节系统之一是葡萄球菌中的SarA蛋白家族。在所有已测序的S.金黄色葡萄球菌和epidermidis基因组这些蛋白质调节许多参与调控、毒力、自溶、生物膜形成和代谢过程的基因。该家族基因调控的确切机制及其对靶基因的调控是本实验室的研究重点。 除此之外，我们还在研究金属氧化物纳米颗粒，以开发潜在的药物，控制和防止细菌在社区和医院环境中传播。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Staphylococcus aureus is one of the major animal pathogens that continues to be a main public health concern; due to the lack of an effective vaccine and the emergence of multiple antibiotic-resistant strains either in community associated or in hospital associated. Staphylococcal pathogenesis is a complex multifactorial process, which involves multiple regulatory systems. The function of the regulatory systems or the transcriptional regulation of the basic biological process is based on DNA-protein and protein-protein interactions to the upstream promoter region; therefore, understanding the role of the networks involved in gene regulation will be useful for the development of new agents to control and prevent staphylococcal infections. One of these regulatory systems is the SarA protein family in Staphylococcus. A total of ten SarA paralogs (SarA, SarR-V, SarX and Z, MgrA and Rot) are found almost in all sequenced S. aureus and S. epidermidis genomes. These proteins regulate many genes involved in regulation, virulence, autolysis, biofilm formation and metabolic processes. The precise mechanisms, by which this family of genes are regulated and regulate its target genes, are the research focuses in our laboratory. Apart from these, we are also working on metal oxide nanoparticles to develop potential agents, which will control and prevent the bacterial spreading in the community and hospital settings.