MECHANISMS OF TRANSCRIPTIONAL COORDINATION AMONG PHOSPHORYLASE KINASE GENES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 磷酸化酶激酶(PhK)是糖原分解途径中的关键调节酶，通过磷酸化来催化磷酸化酶的激活。这种具有亚基组成(阿尔法、贝塔、伽马、德尔塔)的十六聚体酶4，用于整合荷尔蒙、代谢和神经信号，导致肌肉和肝脏中糖原储存的分解。PhK的每个亚基都由一个单独的基因编码，所有的亚基都存在多种组织特异性亚型。虽然已经做了很多工作来阐明PhK催化的生物化学调节，但关于这种复杂低聚物的转录调节的信息很少。我们的工作旨在通过对所有人类α、β和伽马启动子的鉴定和功能分析来阐明那些负责PhK基因表达的时间和空间调节的元件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Phosphorylase kinase (PhK) is a key regulatory enzyme involved in the glycogenolytic pathway, catalyzing the activation, by phosphorylation, of phosphorylase. The hexadecameric enzyme, with subunit composition (alpha,beta,gamma,delta)4, serves to integrate hormonal, metabolic and neural signals, leading to the breakdown of glycogen stores in muscle and liver. Each subunit of PhK is encoded by a separate gene, and multiple tissue specific isoforms exist for all of the subunits. While much work has been done to elucidate the biochemical regulation of PhK catalysis, little information regarding transcriptional regulation of this complex oligomer is available. Our work seeks to elucidate those elements responsible for the temporal and spatial regulation of PhK gene expression by providing for the identification and functional analysis of all human alpha, beta and gamma promoters.