TUMOR DIAGNOSTICS BY VIBRATIONAL SPECTROSCOPY

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的总体目标是使振动光谱应用于癌症诊断和治疗监测。 在癌症的最早阶段发现是至关重要的，因为它大大提高了成功治疗的可能性。 传统的诊断方法依赖于物理切除一部分组织和显微镜下的形态评估。 组织去除的需要减少了可以取样的组织面积。 非侵入性技术可以消除这个问题。 此外，非侵入性技术有可能允许在用于诊断的相同内窥镜手术期间开始治疗，并减少与组织去除相关的其他并发症，例如组织处理和患者感染风险增加。 我们将专注于发展拉曼光谱检测与巴雷特食管患者的癌前病变。 巴雷特食管是一种病理学，其中鳞状上皮衬里被特化化生上皮取代，腺癌的可能性增加。 由于不典型增生的显微镜下变化很难观察到，因此应对整个化生上皮区域进行取样。 因此，Barrett食管非常适合于非侵入性诊断技术。 本提案中开发的方法和技术也可应用于其他组织，如子宫颈。 我们工作的第二个目标是发展拉曼光谱作为评估治疗效果的方法。 目前用于监测个体肿瘤对治疗的反应的方法是不可靠的，并且通常难以在治疗过程中实施。 发展非侵入性或微创光学方法来可靠地识别细胞凋亡和坏死的区域，将为测定每个癌症患者的肿瘤反应提供一种简单的方法。 因此，可以定制治疗。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our general objective is to enable the application of vibrational spectroscopy to cancer diagnosis and treatment monitoring. The detection of cancer at its earliest stages is crucial, for it greatly improves the likelihood of successful treatment. Traditional methods of diagnosis have relied on physical removal of a portion of tissue and microscopic assessment of morphology. The need for tissue removal reduces the area of tissue that can be sampled. A noninvasive technique would eliminate this problem. Furthermore, a noninvasive technique has the potential to allow treatment to begin during the same endoscopic procedure used for diagnosis and reduce other complications associated with tissue removal such as tissue handling and increased risk of infection to the patient. We will focus on developing Raman spectroscopy for detection of precancerous conditions in patients with Barrett's esophagus. Barrett's esophagus is a pathology in which the squamous epithelial lining is replaced by a specialized metaplastic epithelium and the likelihood of adenocarcinoma is increased. Because the microscopic changes of dysplasia are difficult to observe, the entire area of metaplastic epithelium should be sampled. Therefore, Barrett's esophagus is well-suited for a noninvasive diagnostic technique. The methods and techniques developed in this proposal may also find application in other tissues such as the cervix. The second goal of our work is to develop Raman spectroscopy as a method for assessing the effects of treatment. Current methods for monitoring the response of an individual tumor to therapy are unreliable and often difficult to implement during the course of therapy. Development of noninvasive or minimally-invasive optical methods to reliably identify regions of apoptosis and necrosis would provide a simple method for assaying tumor response in each individual cancer patient. Consequently, treatments could be customized.