THREE-DIMENSIONAL STRUCTURE AND FUNCTION OF THE MAMMALIAN KINETOCHORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 哺乳动物动粒是一种蛋白质复合体，在有丝分裂过程中执行四个重要功能：1)将染色体附着在有丝分裂纺锤体上；2)控制动粒微管的动态；3)产生染色体对齐的力量；4)产生一个细胞周期检查点，延迟后期的开始，直到所有的染色体都附着在有丝分裂纺锤体上并在纺锤体赤道上排列。动点和微管之间复杂的相互作用在所有这些重要过程中都是必不可少的。尽管在识别动粒的分子组成方面取得了快速进展，但对动粒功能及其与微管相互作用的潜在机制的了解才刚刚开始。断层重建和连续切片电子显微镜的结果在推断关键分子成分如Hec1(Ndc80)复合体的功能方面发挥了重要作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The mammalian kinetochore is a proteineous complex that performs four vital functions during mitosis: 1) attaching chromosomes to the mitotic spindle; 2) controlling the dynamics of kinetochore microtubules; 3) generating force for chromosome alignment; and 4) generating a cell cycle checkpoint that delays anaphase onset until all chromosomes are attached to the mitotic spindle and aligned at the spindle equator. Intricate interactions between kinetochores and microtubules (MTs) are essential for all of these vital processes. Despite rapid progress in identifying molecular components of the kinetochore, understanding of the underlying mechanisms of kinetochore function and its interactions with microtubules is just beginning to unfold. Results from tomographic reconstructions and serial section electron microscopy are playing a significant part in deducing the function of key molecular components such as the Hec1 (Ndc80) complex.