PROTEIN STRUCTURE DETERMINATION USING CROSS-LINKING, MASS SPECTROMETRY, MODELING

这个子项目是众多研究子项目之一

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We developed a method to determine low resolution structures of proteins from distance constraints derived from mass spectrometry and chemical cross-linking. Although x-ray crystallography and NMR generate high resolution structures, they have limitations: the demand for diffracting crystals, in the first case, and relatively low molecular weight proteins, in the latter. Our new methodology does not have these limitations. We have used MidasPlus and Chimera to help generate homology-modelled structures (using the command swappaa) and to display such structures.