REGULATION OF KINETOCHORE-MICROTUBULE ATTACHMENT BY THE NDC80 COMPLEX

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 忠实的有丝分裂染色体分离对于预防非整倍体细胞至关重要，非整倍体细胞含有异常数量的染色体，并且存在于几乎所有的人类实体肿瘤中。了解真核细胞如何平均分配染色体对于进一步了解癌细胞生物学至关重要。染色体通过有丝分裂纺锤体分离，纺锤体是一种由动态微管阵列组成的复杂装置，需要中心体、动粒和沿着纺锤体微管的许多蛋白质的功能。有丝分裂的一个关键步骤是染色体附着在纺锤体上。染色体通过它们的着丝粒附着在纺锤体微管上，着丝粒是位于有丝分裂染色体的初级缢痕或着丝粒区域的大型蛋白质结构。正是在动粒处，微管必须与动粒保持持久的连接，并在整个有丝分裂过程中保持连接，以排列和分离染色体。异源四聚体Ndc 80复合物已被证明在许多细胞类型是必不可少的着丝粒微管附着。我们的目标是确定与Ndc 80复合蛋白相互作用的蛋白质，以协调调节有丝分裂中的着丝粒微管附着。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Faithful mitotic chromosome segregation is essential in the prevention of aneuploid cells, which contain an abnormal number of chromosomes and are present in almost all human solid tumors. Understanding how eukaryotic cells distribute their chromosomes equally is essential in furthering the understanding of cancer cell biology. Chromosomes are segregated via the mitotic spindle, a complex apparatus that consists of a dynamic array of microtubules, and requires the function of numerous proteins at centrosomes, kinetochores, and along spindle microtubules. A critical step in mitosis is attachment of chromosomes to the mitotic spindle. Chromosomes attach to spindle microtubules via their kinetochores, which are large proteinaceous structures located at the primary constriction, or centromere region, of mitotic chromosomes. It is here at the kinetochore where persistent attachments must be made to microtubules and maintained throughout mitosis to align and segregate chromosomes. The heterotetrameric Ndc80 complex has been demonstrated in many cell types to be essential for kinetochore-microtubule attachment. We aim to identify proteins that interact with Ndc80 complex proteins to coordinately regulate kinetochore-microtubule attachment in mitosis.