CRYSTALLOGRAPHIC STUDIES OF SIGNALING PROTEINSC

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们计划收集两种晶体用于数据收集。第一个是CA 150的FF结构域。我们在ALS收集了一些衍射数据，但由于衍射极限不足，无法解析结构。 我们计划从这次旅行中收集至少一个Se-MAD数据集到3.5 º。 第二种晶体是一种肿瘤抑制蛋白，其表达在卵巢癌和乳腺癌细胞中丢失或显著下调。ARHI蛋白属于Ras超家族，但它具有独特的N-末端延伸，这使得其在正常细胞中具有生长抑制功能。我们已经使蛋白质结晶，它们在家里衍射到~3.9 º。我们计划至少收集一个本地数据集，如果我们能在旅行前得到晶体，可能还有一个Se-MAD。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We plan to collect bring two kinds of crystals for data collection. The first is the FF domain of CA150. We collected some diffraction data at ALS but were not able to solve the structure, due to insufficient diffraction limit. We plan to collect at least a Se-MAD dataset to 3.5 ¿ from this trip. The second kind of crystals were of a tumor supressor protein whose expression is either lost or dramatically down-regulated in ovarian and breast cancer cells. The protein, ARHI, belongs to the Ras super family but it has a unique N-terminal extension which renders its function in growth inhibition in normal cells. We have crystallized the protein and they diffracted to ~3.9 ¿ at home. We plan to collect at least a native dataset and possibly a Se-MAD if we can getthe crystals before the trip.