ANALYSIS OF CELL MIGRATION IN AN AXOLOTL SKIN EXPLANT WOUND MODEL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们建立了一种外植体皮肤模型来研究Axolotl皮肤的伤口愈合。使用传统显微镜，我们已经证实，一个2毫米的伤口在10小时内就能愈合。我们正在使用LFD来解决以下问题。 1)重塑实验伤口床的细胞的来源是什么？它们是起源于表皮的基细胞还是超基细胞？ 2)细胞迁移的速度和方向是什么？这种情况在迁徙的表皮中有变化吗？底物对迁移有影响吗？

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed an explant skin model to study wound healing in axolotl skin. Using conventional microscopy we have established that a 2 mm wound repithelializes in less than 10 hours. We are using the LFD to address the following questions. 1) What is the origin of the cells that repithelialize the experimental wound bed? Are they derived from basal or suprabasal cell in the epidermis? 2) What is the rate and direction of cell migration? Does this vary within the migrating epidermis? Does the substrate affect migration?