STRUCTURE AND DYNAMICS OF 6-THIOGUANINE MODIFIED DNA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 硫嘌呤的毒性是在代谢后以6-硫代脱氧鸟苷的形式并入DNA后产生的。硫代脱氧鸟苷和/或其代谢产物甲基-6-硫代脱氧鸟苷改变了DNA/蛋白质的重要相互作用，包括DNA错配修复、拓扑异构酶II、端粒酶和核糖核酸酶H。我们正在使用溶液核磁共振光谱来表征6-硫代鸟苷修饰的寡核苷酸中局部DNA结构和动力学的变化

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Toxicity of thiopurines results after being metabolized and incorporated into DNA as 6-thiodeoxyguanosine. The alteration of local DNA structure by thiodeoxyguanosine and/or its metabolite methyl-6-thiodeoxyguanosine alters important DNA/protein interactions including DNA-mismatch repair, topoisomerase II, telomerase, and RNase H. We are using solution NMR spectroscopy to characterize the changes in local DNA structure and dynamics in 6-thioguanine modified oligonucleotides