QUANTIFICATION OF 13C-OPTIMIZED CRYOGENICALLY-COOLED PROBES FOR CHEM RES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 进行测试以量化低温冷却探针用于化学研究的效用，特别是在获得直接检测的碳-13数据方面。 高度不饱和化合物是威斯康星大学麦迪逊分校化学系各种研究项目的合成目标。 这些化合物有时很难用逆（质子检测）方法表征，现在常见的复杂化合物的NMR分析。 不依赖于1H-13 C J-偶联存在的总体表征的优秀技术，如INADEQUATE，过去需要13 C标记或非常高的浓度。 使用低温冷却探针获得中等浓度天然丰度化合物的此类数据的能力，其中13 C前置放大器也被冷却以降低其噪声系数，将使用NMRFAM的冷冻探针在750 MHz下进行测试。 还将量化探针用于改善1D 13 C数据采集的实用性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Tests are performed to quantify the utility of cryogenically-cooled probes for chemical research, especially with respect to obtaining directly-detected carbon-13 data. Highly unsaturated compounds are synthetic targets for a variety of research projects in the Chemistry Department at the UW-Madison. These compounds are sometimes difficult to characterize using inverse (proton-detected) methods now common with NMR analysis of complex compounds. Excellent techniques for total characterization that do not rely on the presence of 1H-13C J-couplings, such as INADEQUATE, have in the past required either 13C labeling or very high concentrations. The ability to obtain such data for moderately concentrated natural abundance compounds using a cryogenically-cooled probe, with the 13C preamp also cooled to reduce its noise factor, will be tested using NMRFAM's cryoprobe at 750 MHz. The utility of the probe for improving the acquisition of 1D 13C data will also be quantified.