STRUCTURAL STUDY OF PHI 29 PRNA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 枯草芽孢杆菌噬菌体<$29的前导RNA（pRNA）是基因组最左端的174个碱基的转录物，是<$29 DNA包装马达的重要组成部分。 一种新的pRNA多聚体与前体蛋白壳的头尾连接体（prohead）结合，随后ATP酶gp 16与pRNA的结合构成了转位19个ATP酶对DNA-基因产物3复合物（DNA-gp 3）的马达。 ATP水解驱动DNA-gp 3包装，一个ATP用于包装两个碱基对的DNA。 pRNA的发现29，病毒DNA包装机制研究的主要系统，具有普遍意义，因为共同的机制可用于包装尾噬菌体和某些动物病毒的双链DNA。pRNA作为DNA包装中的瞬时结构组分和催化剂的作用扩展了RNA的新结构和生物化学功能的演变。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Prohead RNA (pRNA) of the Bacillus subtilis bacteriophage ¿29, a 174-base transcript of the far left end of the genome, is an essential component of the ¿29 DNA packaging motor. A novel pRNA multimer binds to the head-tail connector of the precursor protein shell (prohead), and subsequent binding of the ATPase gp16 to pRNA constitutes the motor that translocates the 19 kilobase pair DNA-gene product 3 complex (DNA-gp3). ATP hydrolysis drives DNA-gp3 packaging, and one ATP is used to package two base pairs of DNA. The discovery of pRNA in ¿29, the leading system for mechanistic studies of viral DNA packaging, has general significance in that common mechanisms may be used in packaging double-stranded DNAs of the tailed bacteriophages and certain animal viruses. The role for pRNA as a transient structural component and catalyst in DNA packaging extends the evolvement of novel structural and biochemical functions for RNA.