MECHANISMS OF PROTEIN AND RNA FOLDING AT HIGH RESOLUTION

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目标是通过核磁共振以高分辨率深入了解蛋白质和RNA折叠的基本机制。蛋白质折叠子项目旨在剖析链长如何影响溶液中小的单结构域蛋白质的特定构象特征。 将在不存在和存在凝聚活性分子伴侣的情况下进行研究。N-末端模型肽片段的骨架分配和构象将通过双共振和三共振NMR进行研究。RNA折叠子项目解决了一些基本问题，如在小的生物相关模型RNA序列中存在动力学/平衡中间体和碱基对形成途径。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this research is to gain insights into the fundamental mechanisms governing protein and RNA folding at high resolution by nuclear magnetic resonance. The protein folding subproject aims at dissecting how chain length affects the specific conformational features of small single domain proteins in solution. The studies will be performed in the absence and presence of cotranslationally active molecular chaperones. Backbone assignments and conformatiion of N-terminal model peptide fragments will be studied by double and triple resonance NMR. The RNA folding subproject addresses fundamental issues such as the presence of kinetic/equilibrium intermediates and base pair formation pathways in small biologically relevant model RNA sequences.