SINGLE MOLECULE ELECTRICAL RECORDING WITH NANOPORE DEVICE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 纳米尺寸的孔，所谓的纳米孔，可以在薄的无机膜中制造。纳米孔最重要的应用是DNA测序：在电场的影响下，DNA通过纳米孔移位，产生DNA链的序列和长度特征的电信号。 目前的合成纳米孔还不能达到单碱基分辨率;然而，它们是最有前途的廉价DNA测序技术之一。 该资源一直在与电气工程师（格雷戈里·蒂普和让-皮埃尔·勒伯顿）密切合作，以了解合成纳米孔的物理学并提高其分辨率。 在三个方向上进行原子级建模：（i）用合成纳米孔进行基因分型;（ii）用合成纳米孔拉伸/解压缩DNA发夹;（iii）用纳米孔电容器感测DNA序列;（iv）对通过二氧化硅纳米孔的离子电流进行建模（http：//www.ks.uiuc.edu/Research/nanopore/）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A nano-meter size pore, a so-called nanopore, can be manufactured in thin inorganic membranes. The most important application for nanopores is DNA sequencing: under the influence of an electric field, DNA translocates through the nanopore, producing electrical signals characteristic of the sequence and length of the DNA strand. Current synthetic nanopores can not reach single-base resolution yet; however, they are among the most promising technologies for cheap DNA sequencing. The Resource has been working in close collaboration with electrical engineers (Gregory Timp and Jean-Pierre Leburton) to understand the physics of synthetic nanopores and improve their resolution. Atomic-scale modeling was carried out in three directions: (i) genotyping with synthetic nanopore; (ii) stretching/ unzipping DNA hairpins with a synthetic nanopore; (iii) sensing DNA sequence with a nanopore capacitor; (iv) modeling of ionic current through silica nanopores (http://www.ks.uiuc.edu/Research/nanopore/).