EM STUDY OF THE AUTOPHAGY PATHWAY AFTER BRAIN ISCHEMIA

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是探讨短暂性脑缺血再灌注后自噬通路的损伤。我们的方法将依赖于光学和电子显微镜的组合来评估缺血对自噬途径和相关蛋白的影响。特别是宽场电子断层扫描的使用应该显着提高我们可以检查组织样本的效率，从而增加这项研究的力量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this proposal is to investigate impairment of the autophagy pathway after transient cerebral ischemia followed by reperfusion. Our approach will rely on a combination light and electron microscopy to assess the effect of ischemia on the autophagy pathway and associated proteins. The use of wide-field electron tomography in particular should significantly increase the efficiency with which we can examine tissue samples and thereby increase the power of this study.