STRUCTURE-FUNCTION STUDIES OF YEAST RNR1

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 核糖核苷酸还原酶（RNR）催化从头DNA合成中的限速步骤，并且通过dNTP的相对水平变构控制以维持细胞中脱氧核苷酸三磷酸（dNTP）的平衡池。 在真核生物中，该酶包含a2和b2亚基的异源寡聚体。a亚基（Rnr 1）含有调节和催化位点; Rnr 2（B亚基）含有对催化至关重要的二铁酪氨酰自由基。严格控制dNTP池对细胞活力至关重要;由于RNR在平衡dNTP的相对水平方面起着至关重要的作用，它通过细胞内各种亚基的区室化在转录、变构和S.酿酒酵母通过其蛋白抑制剂Sml 1。这些过程的分子基础尚未完全了解。RNR对快速分裂的细胞至关重要，是癌症治疗的靶点

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ribonucleotide reductase (RNR) catalyzes the rate-limiting step in de novo DNA synthesis and is allosterically controlled by relative levels of dNTPs to maintain a balanced pool of deoxynucleotide triphosphates (dNTPs) in the cell. In eukaryotes, the enzyme comprises a hetero-oligomer of a2 and b2 subunits. The a subunit (Rnr1) contains regulatory and catalytic sites; Rnr2, the b subunit, houses the diferric-tyrosyl radical crucial for catalysis. Tight control of dNTP pools is vital for cell viability; due to the crucial role RNR plays in balancing the relative levels of dNTPs, it is highly regulated transcriptionally, allosterically, by compartmentalization of the various subunits within the cell, and in S. cerevisiae by its protein inhibitor Sml1. The molecular basis for these processes is not fully understood. Crucial for rapidly dividing cells, RNR is a target for cancer therapy