Characterization of the Brucella virB locus

在此输入文本，它是应用程序的新摘要信息。本节必须是否 VII 超过30行的文本。 人布鲁氏菌病，由布鲁斯/Ia属引起，是全球最广泛的人畜共患疾病之一，估计每年有50万新病例。一个 B重要方面。流产感染是其在网状内皮系统的吞噬细胞内持续存在的能力。我们已经表明，virB基因座，编码IV型分泌系统（T4SS），是必不可少的吞噬细胞和小鼠的毒力的生存。然而，T4 SS允许B.在宿主中建立其生态位的能力尚不清楚。本申请的目的是表征由B分泌的分子的作用。流产T4SS与宿主细胞的相互作用。本申请的中心假设是，T4SS在宿主中建立复制生态位中起作用， 可以D-0 O-0 0 fl） e （fl 将含布鲁斯//a的空泡靶向内质网（ER）。拟议研究的基本原理是， v，' 良好的研究环境，非常有利于其顺利完成。将通过追求以下具体目标来检验中心假设并实现本申请的目的：（i）确定T4SS易位效应蛋白对网状内皮系统持续感染的贡献 显著影响驱动该领域的概念和方法。

Enter the text here that is the new abstract information for your application. This section must be no vii longer than 30 lines of text. Human brucellosis, caused by Bruce/Ia spp., is one of the most widespread zoonotic diseases globally, with an estimated 500,000 new cases each year. An important aspect of B. abortus infection is its ability to persist within phagocytic cells of the reticuloendothelial system. We have shown that the virB locus, encoding a Type IV secretion system (T4SS), is essential for survival in phagocytes and virulence in mice. However, the mechanisms by which the T4SS allows B. abortus to establish its niche in the host are unknown. The objective of this application is to characterize the role of molecules secreted by the B. abortus T4SS in interaction with host cells. The central hypothesis of this application is that the T4SS functions in establishing a replicative niche in the host by can D-0 O-0 0 fl) E (fl targeting the Bruce//a-containing vacuole to the endoplasmic reticulum (ER). The rationale for the proposed research is that characterization of virulence mechanisms v,' excellent research environment that is highly conducive to its successful completion. The central hypothesis will be tested and the objectives of this application accomplished by pursuing the following specific aim: (i) Determine the contribution of T4SS-translocated effector proteins to persistent infection of the reticuloendothelial system significantly influencing concepts and methods driving the field.