Analytical Cytometry

OSUCCC分析细胞计数共享资源（ACSR）提供了快速准确的方法， 分析生物颗粒的多种特性，同时还能够快速、准确地 高纯度（> 98%）的细胞分选出感兴趣的纯细胞群， 调查员这项服务还为OSUCCC成员提供了获得可行的， 无菌和纯的细胞群，以便它们可以单独克隆，可以评估 免疫功能，或者可以用最少的操作检查特定的生化特性， 与磁珠技术相比。 ACSR有三个主要目标：1）为电池提供仪表和技术操作/支持 OSUCCC成员和大学社区的识别/表征和分离，2） 提供"应用研究"支持，以解决"非典型"或独特/困难的细胞鉴定/分离问题 3）向会员介绍新的细胞鉴定/分离技术和方法 正在OSU和其他机构/组织开发。 ACSR目前有四台流式细胞仪，其中一台是最近购买的， 用最先进的Becton-Dickenson流式分选机进行了升级。另外两种流式细胞仪分析仪是 可用于独立（24小时访问）和操作辅助分析。此外，商业和 可提供Jrototype磁分离和分析设备。 ）过去五年，机场核心走廊的每小时使用量增加了560%，由一九九八年的937.5小时增至超过 2003年为5,251小时。CCSG同行评审资助的OSUCCC成员占总使用量的88% 并代表所有六个OSUCCC科学计划。本策略检讨继续为 科学计划，现在包括临床研究的研究数据，并有助于杰出的科学 癌症研究作为OSUCCC扩大其研究组合。

The OSUCCC Analytical Cytometry Shared Resource (ACSR) provides the means of rapidly and accurately analyzing multiple characteristics of biological particles while also being able to rapidly, accurately, and with high purity (>98%) sort out pure populations of cells of interest based on parameters designated by the investigator. This service furthermore provides the OSUCCC members with the ability to obtain viable, sterile, and pure populations of cells so that they may be individually cloned, can be assessed for immunological function, or can be examined for specific biochemical properties with minimal manipulations, compared to magnetic bead technologies. The ACSR has three primary goals: 1) Provide instrumentation and technical operation/support for cell identification/characterization and separation to OSUCCC members and the university community, 2) Provide "applied research" support to address "atypical" or unique/difficult cell identification/separation problems, 3) Introduce the membership to new cell identification/separation technology and methodologies that are being developed at OSU and other institutions/organizations. The ACSR currently has four flow cytometry instruments, one of which was recently purchased and upgraded with a state-of-the-art flow sorter from Becton-Dickenson. Two other flow cytometer analyzers are available for independent (24 hour access) and operated-assisted analysis. In addition, commercial and Jrototype magnetic separation and analysis equipment is available. )ver the last five years, hourly usage of the ACSR has increased 560%, from 937.5 hours in 1998 to over 5,251 hours in 2003. CCSG peer-reviewed funded OSUCCC members account for 88% of the total usage and represent all six OSUCCC scientific programs. This ACSR continues to provide critical support to the scientific programs, now including research data on clinical studies, and contributes to outstanding scientific cancer research as the OSUCCC expands its research portfolio.