CORE--HISTOLOGY

核心C是资源核心。这三个项目都将广泛依赖于对小鼠的组织学分析 红心。集中设备、技术专长和试剂将便利这些活动和 最大限度地降低相关成本。核心C的具体活动包括(1)协助常规石蜡 包埋和切片，(2)协助常规冷冻保存和切片，(3)协助 标准组织化学染色(即苏木精伊红、阿尔新蓝、马森氏三色、天狼星红和 Fast Green、von Kossa等)，(4)协助原位杂交，以及(5)协助常规免疫 组织学分析(如TUNEL、BrdU、肌节肌动蛋白、肌球蛋白重链等)。考虑到沉重的 所有三个项目对组织学分析的利用，核心C对于成功完成 拟议的实验。

Core C is the Resources Core. All three Projectswill rely extensively on histological analysis of mouse hearts. Centralization of equipment, technical expertise and reagents will facilitate these activities and minimize the associated costs. Specific activities of Core C include (1) assistance with routine paraffin embedding and sectioning, (2) assistance with routine cryopreservation and sectioning, (3) assistance with standard histochemical staining (i.e., Hematoxylin and Eosin, Alcian blue, Masson's trichrome, Sirius red and fast green, von Kossa, etc), (4) assistance with in situ hybridizations, and (5) assistance with routine immune histology analyses (i.e., TUNEL, BrdU, sarcomeric actin, myosin heavy chain, etc). Given the heavy utilization of histologic analyses by all three projects, Core C is essential for the successful completion of the proposed experiments.