Core--Animal Model, Analytical Chemistry and Biochemistry

?大鼠高血压实验模型的建立。反映PPG的重点是 临床相关的细胞离子事件和它们在肌源性血管张力中的作用，选择的模型将 哇巴因高血压大鼠这种高血压的诱导形式沿着匹配的对照， 提供各种血管制备物的来源，包括完整的动脉和血管细胞。 ?血液循环中内源性和外源性哇巴因（和其他化合物）的测量 动物 ?大鼠和小鼠小动脉急性分离肌细胞的制备。 ?转基因小鼠的培育和基因分型。 核心的主要目的有三个方面。首先，它将为广泛使用提供新的和/或独特的工具 PPG的各种项目。所提供的每一种工具和资源都与 PPG项目的目标。因此，该核心将通过提供有关 常规基础，否则将证明是繁重的和/或超出个人的专业知识 PPG的项目。第二，该核心将负责协调项目编制补助金项目的活动， 各种试剂和制剂可以通过不同的方法同时进行研究（适当时） 在每个PPG项目中。第三，核心将促进工具的最高效生成和使用 PPG研究者 PPG内有相当多的经验-所有研究者都有非常广泛和发表的 建议的各种血管准备的专业知识。哈姆林博士是创始人之一（与博士。 Yuan，Pamnani和Haddy）的高血压大鼠模型。他有十多年的 实践经验的模型（超过任何其他研究者在世界各地），是唯一的个人 在PPG中具有详细的模型范例知识和经验。戈洛什博士有广泛的 细胞和分子方法的经验。

? Generation of an experimental model of hypertension in the rat. Reflecting the focus of the PPG on clinically relevant cellular ionic events and their role in myogenic vascular tone, the model of choice will be the ouabain hypertensive rat. This induced form of hypertension along with matched controls will provide a source of various vascular preparations including intact arteries and vascular cells. ? Measurement of endogenous and exogenous ouabain (and other compounds) in the circulation of animals. ? Preparation of acutely dissociated myocytes from rat and mice arterioles. ? Breeding and genotyping of transgenic mice. The primary purpose of the Core is three fold. First it will provide new and/or unique tools for extensive use by the various projects of the PPG. Each of the tools and resources to be provided is directly related to the aims of the PPG projects. As such, the core will serve an essential role by providing tools and reagents on a routine basis that would otherwise prove to be burdensome and/or beyond the expertise of the individual projects of the PPG. Second, the core will serve to coordinate the activities of the PPG projects so that the various reagents and preparations can be studied (when appropriate) simultaneously by different methods in the each of the PPG projects. Third, the core will promote the most efficient generation and use of tools by the PPG investigators Considerable experience exists within the PPG - all investigators have very extensive and published expertise with the various vascular preparations proposed. Dr. Hamlyn is one of the originators (with Drs. Yuan, Pamnani and Haddy) of the hypertensive rat model used in the program. He has over ten years of hands on experience with the model (more than any other investigator worldwide) and is the only individual in the PPG with detailed knowledge and experience of the model paradigm. Dr. Golovina has extensive experience with the cellular and molecular methods.