Imaging & microscopy

成像和显微镜核心的主要目标是通过提供 获得成像和显微镜设备，这些设备在 没有专门研究这些技术的个人研究人员的实验室。这使调查人员能够扩大 在神经科学研究所内，可用于他们研究的技术允许 个人调查人员、神经科学研究所和莫尔豪斯学院分担的费用 医学部。 成像核心可为明场、荧光、共聚焦显微镜和成像提供设备 收购。硬件和软件被设计成允许产生和分析相关的图像 通过原位杂交、免疫细胞化学、组织学以及凝胶的数字化和分析 由北方、西部、南方、聚合酶链式反应和逆转录-聚合酶链式反应等分子技术产生。图片 所生产的是出版物的质量。 在U54参与者中，戴维森实验室将使用低温恒温器和显微镜成像 用于对轮班小鼠采集的组织样本进行病理分析的核心设备 轻松的日程表。保罗实验室将使用成像核心中的低温恒温器对脑组织进行原位切片 放射自显影图像的杂交和随后的光密度分析。此外，保罗 实验室将使用共聚焦显微镜辅助绘制GABAA亚单位在 使用新型GABAA亚单位抗体的视交叉上核和其他下丘脑核团。这个 福原实验室将使用Brightfield和荧光显微镜检查Bmall-荧光素酶 利用原位杂交和图像分析软件成功地将DNA传递给成纤维细胞 由核心提供。核心设备也可以在其他神经科学的适当指导下使用 研究所调查人员和莫尔豪斯医学院的其他人员。

The major aim of the Imaging and Microscopy Core is to facilitate the research of investigators by providing access to imaging and microscopy equipment that might be expensive to procure and maintain in an individual investigator's laboratory not dedicated to these techniques. This allows investigators to broaden the techniques available for their research within the Neuroscience Institute by allowing the burden of expenses to be shared between individual investigators, the Neuroscience Institute and Morehouse School of Medicine. The imaging core can provide equipment for brightfield, fluorescence and confocal microscopy and image acquisition. The hardware and software are designed to allow production and analysis of images associated with in situ hybridization, immunocytochemistry, histology, as well as digitization and analysis of gels produced from molecular techniques such as Northerns, Westerns, Southerns, PCR and RT-PCR. Images produced are publication quality. Among the U54 participants, the Davidson laboratory will use the cryostat and microscope imaging equipment of the core to perform pathological analyses on tissue samples harvested from mice on shifting light schedules. The Paul laboratory will use the cryostat in the imaging core to section brain tissue for in situ hybridization and subsequent optical density analysis of autoradiograph images. In addition, the Paul laboratory will use confocal microscopy to assist in mapping expression of the GABAA subunit expression in the suprachiasmatic nucleus and other hypothalamic nuclei using novel GABAA subunit antibodies. The Fukuhara laboratory will use brightfield and fluorescence microscopy to examine whether Bmall-luciferase DNA is successfully delivered to fibroblasts utilizing in situ hybridization and image analysis software provided by the core. Core equipment can also be used, under proper guidance by other Neuroscience Institute investigators and other Morehouse School of Medicine personnel.