Role of Intronic Variants Affecting Splicing in Juvenile Myoclonic Epilepsy

描述（由申请人提供）：青少年肌阵挛性癫痫（JME）是特发性全身性癫痫（IGE）最常见的综合征类型之一，具有复杂的基因遗传。虽然已经确定了几个假定的JME易感基因，但这些基因在大脑中的功能及其在癫痫发作发病机制中的作用尚不清楚。连锁和关联分析使我们找到了含溴结构域的基因BRD2。BRD2是调控基因表达的双溴结构域基因BET亚家族的成员。我们已经证明BRD2在发育和成人大脑中表达，最近，BRD2缺失的胚胎在妊娠中期死亡，伴有中枢神经系统畸形。此外，我们的初步数据显示，杂合Brd2小鼠表现出额叶皮层gaba能中间神经元数量的减少，并且，引人注目的是，杂合Brd2小鼠对化学诱导的癫痫发作表现出增强的敏感性。我们的工作假设是BRD2的错误表达有助于癫痫易感性。我们还发现BRD2的内含子2包含一个高度保守的、可选择性剪接的外显子，该外显子引入了一个过早终止密码子，我们已经确定了内含子2中ca重复序列长度的多态性，这些多态性与JME相关。在其他实验模型中，ca重复序列长度的变化已被证明会影响选择性剪接事件。我们现在希望验证这样一个假设，即内含子2的多态性，特别是我们在患者DNA中发现的与JME相关的CA重复长度的变异，会影响备选外显子的剪接和相应转录本的水平，从而影响BRD2产物的功能。在Aim 1中，我们将首先通过瞬时表达含有人类BRD2的[外显子2]-[内含子2]-[外显子3]的“迷你基因”构建体，并测量产生的两个转录本的比例，研究可变ca重复长度对两种选择性剪接BRD2转录本相对水平的影响。在目标2中，我们将通过确定替代转录本是否被翻译，以及如果被翻译，所产生的蛋白质是否影响同二聚化或亚细胞定位，开始阐明替代剪接转录本的功能意义。

DESCRIPTION (provided by applicant): Juvenile Myoclonic Epilepsy (JME) is among the most common types of syndromes of Idiopathic Generalized Epilepsy (IGE) and has a complex genetic inheritance. Although several putative JME susceptibility genes have been identified, the functions of these genes in the brain and their roles in the pathogenesis of seizures are unknown. Linkage and association analysis led us to the bromodomain-containing gene BRD2. BRD2 is a member of BET subfamily of double bromodomain-containing genes implicated in regulating gene expression. We have shown that BRD2 is expressed in developing and adult brain, and recently, that Brd2-null embryos die at mid-gestation with central nervous system malformations. Further, our preliminary data show that heterozygous Brd2 mice exhibit a reduction in the number of GABAergic interneurons in the frontal cortex and, strikingly, heterozygous Brd2 mice exhibit an enhanced sensitivity to chemically-induced seizures. Our working hypothesis is that mis-expression of BRD2 contributes to seizure susceptibility. We have also discovered that intron 2 of BRD2 contains a highly conserved, alternatively spliced exon, which introduces a premature termination codon and we have identified polymorphisms in the lengths of CA-repeats in intron 2 that are associated with JME. Variation in the length of CA-repeats has been shown to affect alternative splicing events in other experimental models. We now wish to test the hypothesis that the polymorphisms in intron 2, in particular variants in CA repeat-length that we have identified in patient DNA that associate with JME, affect splicing of the alternative exon and the levels of the corresponding transcripts, and hence, function of BRD2 products. In Aim 1, we will initially examine the effect of variable CA-repeat lengths on the relative levels of the two alternatively spliced BRD2 transcripts by transient expression of 'mini-gene' constructs containing [exon 2]-[intron 2]-[exon3] of human BRD2 with varying CA-repeat lengths and measuring the ratio of the resulting two transcripts. In Aim 2, we will begin to elucidate the functional significance of the alternatively spliced transcripts by determining whether the alternative transcripts are translated and if so, whether the proteins made affect homodimerization or sub-cellular localization. PUBLIC HEALTH RELEVANCE: Juvenile Myoclonic Epilepsy (JME) is among the most common types of syndromes of Idiopathic Generalized Epilepsy (IGE) and is characterized by adolescent onset and life-long affliction, requiring continual medication to suppress seizures. The proposed experiments will demonstrate whether the JME- associated variants in intron 2 of BRD2 play a direct role in the frequency of alternative splicing, and further, will provide insight into the possible physiological significance of the alternative transcripts, outcomes that are highly relevant to the stated goals of the RFA and the R03 funding mechanism.