La Jolla Interdisciplinary Neuroscience Center Cores - Electro Core BU

电生理学核心 1.主要举措和新方向 电生理学核心将是一个新的设施。目前，没有统一的电生理学 连接拉霍亚托里松树梅萨的神经科学家的核心设施。因此本 电生理学设施将构成一个新的核心，以促进神经科学家之间的合作 在圣地亚哥地区。 了解神经系统是如何工作的需要电生理技术来获得 了解突触活动是如何被调节和调制的。电生理学的核心是 神经科学蓝图计划旨在使电生理学作为一种研究工具， 托里派恩斯梅萨上的大量神经科学家。在过去的几年里，科... 这个核心的董事，博士斯蒂芬F。Helnemann和Charles F.史蒂文斯成为少校 有助于理解两个主要的兴奋性递质系统的功能， 烟碱乙酰胆碱和谷氨酸受体系统。他们的工作提供了重要的见解， 突触传递和突触可塑性的机制。两位研究人员都做出了贡献。 他们的知识和努力，与神经科学家谁没有直接的合作， 专业知识或电生理分析的实验装置。虽然这些合作 取得了重大的研究进展，研究人员总是依赖于是否 主机实验室可以在仪器可用性方面容纳额外的实验， 人员的随着发展和系统对电生理学的需求日益增加， 神经生物学家，这是及时填补电生理学的额外能力的需要。到 实现这一目标，并进一步增加神经科学家之间的合作， Torrey Pines梅萨是一个新的电生理学核心设施，将作为 神经科学蓝图计划。斯蒂芬·海涅曼博士和查尔斯·史蒂文斯博士将担任 共同董事为这一核心，并致力于促进电生理设计 核心用户的实验。电生理学核心有两个目标：第一，提供 神经科学家在索尔克进行电生理实验的专业知识和仪器 研究所，UCSD，伯纳姆生物医学研究所（BIMR）和斯克里普斯研究所 研究所;第二，通过对话和合作， 这些研究所的科学家。 电生理学核心增加了一个新的研究维度，将提供工具和 专门知识的功能和活动依赖的研究，旨在获得知识的 在神经元中发生的活动依赖性变化，无论是内在的还是响应外部的 信号.主要用户提出的研究项目要求研究神经元活动， 脑切片和/或原代神经元培养物。此外，该核心将与核心相间， 病毒载体/siRNA和用于分子成像核心以测试荧光标记的野生型和 突变蛋白在脑切片和原代细胞中异位表达后的神经元功能。

ELECTROPHYSIOLOGY CORE 1. MAIN OBJECTIVES AND NEW DIRECTIONS The Electrophysiology Core will be a new facility. Currently, there is no unified Electrophysiology Core Facility to link neuroscientists on the La Jolla Torrey Pines Mesa. Therefore, this Electrophysiology facility will constitute a new Core to foster collaborations among neuroscientists in the San Diego area. Understanding how the nervous system works requires electrophysiological techniques to gain insights into how synaptic activity is regulated and modulated. The Electrophysiology Core of this Neuroscience Blueprint Initiative aims to make available electrophysiology as a research tool to a wide number of neuroscientists on the Torrey Pines Mesa. In the past several years, the Co- Directors of this Core, Dr. Stephen F. Helnemann and Dr. Charles F. Stevens made major contributions to understanding the functions of the two major excitatory transmitter systems, the nicotinic acetylcholine and glutamate receptor systems. Their work provided major insights into mechanisms of synaptic transmission and synaptic plasticity. Both researchers have contributed their knowledge and effort to a series of collaborations with neuroscientists who do not have direct expertise or the experimental set-up for electrophysiological analyses. While these collaborations accomplished significant research advancements, investigators always depended on whether the host laboratory could accommodate additional experiments in terms of instrument availability and personnel. With the increasing need for electrophysiology by developmental and systems neurobiologists, it is timely to fill the need for additional capacity in electrophysiology. To accomplish that and to further increase collaborative ventures between neuroscientists on the Torrey Pines Mesa, a new core facility for electrophysiology will be created as part of the Neuroscience Blueprint Initiative. Dr. Stephen Heinemann and Dr. Charles Stevens will serve as Co-Directors for this core and are committed to contribute to the design of electrophysiological experiments by the core users. The Electrophysiology Core has two goals: First, to provide expertise and instrumentation for electrophysiological experiments by neuroscientists at the Salk Institute, UCSD, Burnham Institute for Biomedical Research (BIMR) and The Scripps Research Institute; and second, to enhance neuroscience through dialogue and collaboration between the scientists of these institutes. The Electrophysiology Core adds a new research dimension that will provide the tools and know-how for functional and activity-dependent studies designed to acquire knowledge of the activity-dependent changes occurring in neurons either intrinsically or in response to external signals. The research projects proposed by the major users require studies of neuronal activity in brain slices and/or primary neuron cultures. Moreover, this core will inter-phase with the cores for Viral Vectors/siRNA and for Molecular Imaging cores to test fluorescently tagged wild type and mutant proteins in neuronal functions after ectopic expression in brain slices and primary cells.