CHARACTERIZATION OF ANTIGENIC PEPTIDES PRESENTED BY I-AG7

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目的目标是从NOD小鼠身上鉴定由糖尿病致病的MHC II类分子I-Ag7呈递的多肽。研究将同时使用NOD小鼠和来自胰岛素瘤的细胞系。这项研究将扩展到负载、翻译后修饰和外来多肽和蛋白质的挑战的影响。将使用质谱学对这些多肽进行排序和分类，并将继续开发提高灵敏度和分析的技术。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to identify peptides presented by the diabetogenic MHC class II molecule I-Ag7 from NOD mice. Studies will use both NOD mice and cell lines derived from insulinomas. The study will extend to cover the effect of loading, posttranslational modification, and challenge by foreign peptides and proteins. Mass spectrometry will be used to sequence and catalog these peptides and the development of techniques to improve sensitivity and analysis will be pursued.