Electronic Holography for Biomedical Applications Using a Coherent Source

在活体组织中观察细胞的三维运动对于生物医学科学的研究非常重要。然而，当生物样本在实验室准备进行光学显微镜检查时，来自二维培养的丰富的细胞行为信息与自然生理和病理之间的联系缺失。该提案将结合阿伯丁大学在物理科学数字全息术和生物医学科学细胞运动方面的专业知识，建立一个数字全息系统来研究活的生物样本。这将使我们能够可视化细胞在其实际生活环境中的运动，包括快速移动的细胞，例如那些在血流中的细胞，这是目前不可能在高分辨率下观察到的。活体样品的数字全息图可以方便地记录在电子传感器上，然后在计算机中重现。重建的全息图像显示了三维空间中感兴趣的活体样本的原始场景。与具有二维采样机制的传统成像系统相比，新系统为检查活细胞的行为提供了额外的信息。在阿伯丁大学，光学工程小组在光学工程方面拥有超过25年的经验，是世界上专门从事全息成像的领先小组之一(Watson等人，MEAS)。SCI。《技术评论》，第12卷，L9-15,2001，Sun等人，J.Opt.答：纯正的苹果。选择。6 pp703-10,2005)，并建造了最先进的海洋生物电子全息相机之一。医学科学小组在细胞迁移研究方面有十多年的经验，最近在理解伤口愈合中的细胞运动方面做出了重大贡献(赵等人，自然，第442卷，第457-60页，2006)。跳跃？从光学工程学科到生物医学科学，旨在让申请者获得在生物医学科学学科工作的丰富经验，并将物理科学的光学工程思想有效地应用于医学科学，并建立数字/电子全息视频显微镜系统。预计这次跳跃将显著促进阿伯丁大学光学工程和医学科学小组之间的新互动。

Observations of cell movement in three-dimension within living tissues is very important for the research in biomedical sciences. However, the link between the wealth of cell behavioral information from two dimensional cultures and the natural physiology and pathology is missing when biological samples are prepared for examination under light microscope in the laboratory . The proposal will combine the expertise in digital holography of physical sciences and that in cell movement in biomedical sciences at University of Aberdeen and build a digital holographic system to study living biological samples. This will allow us to visualize cell movement in its actual living environment, including fast moving cells, such as those in the blood stream, which is not currently possible to be observed at high resolution. A digital hologram of a living sample can be conveniently recorded on an electronic sensor and then reconstructed in a computer. The reconstructed holographic images show the original scene of the living samples of interests in three dimensional space. The new system provides extra information for examining living cell behaviours compare with the conventional imaging systems with two dimensional sampling mechanisms. At the University of Aberdeen, the optical engineering group has more than 25 years experience in optical engineering and is one of the leading groups in the world specializing in holographic imaging (Watson et al, Meas. Sci. Technol., vol. 12 pp L9-15, 2001, Sun et al, J. Opt. A: Pure Appl. Opt. 6 pp703-10, 2005) and have built one of the most advanced electronic holographic camera for marine biology. The Medical Sciences Group has more than ten years experience in cell migration study and have recently made a significant contribution in understanding cell movement in wound healing (Zhao et al, Nature, vol. 442, pp 457-60, 2006). The ?hopping? from optical engineering discipline to biomedical sciences is aiming to allow the applicant to gain extensive experience of working in biomedical sciences discipline and apply the optical engineering ideas of physical sciences to medical sciences effectively, and to build a digital/electronic holographic video microscopy system. It is expected that the hopping will significantly promote new interactions between groups of Optical Engineering and Medical Sciences at the University of Aberdeen.